Lyve-1 Positive Cells
Lyve-1 Positive Cells - Related Videos
06:46
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Cited by 51 •
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09:53
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We have developed a lentiviral vector that possesses, in addition to the Tat-responsive LTR, the Rev-response element (RRE) that can regulate reporter gene expression in an HIV-1 Tat- and Rev-dependent fashion. The vector permits the specific detection of replicating HIV in living cells via the expression of GFP.
12:19
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Cited by 71 •
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11:22
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06:08
Generating Single Positive CD8 T Cells from Induced Pluripotent Stem Cells
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2025
This video demonstrates an assay to generate tumor antigen-specific induced pluripotent stem cell (iPSC)-derived CD8αβ+ T Cells. Tumor antigen-specific iPSCs are grown on a stromal cell monolayer and differentiated into hematopoietic progenitor cells (HPCs). The HPCs are differentiated into CD4+ CD8+ double-positive T cells using a differentiation medium. These cells are then stimulated to generate single-positive T cells expressing CD8.
FAQs
Lyve-1 expression does not identify a single cell population in every tissue. Lymphatic endothelial cells and certain macrophage populations can both produce the signal, so a Lyve-1 result alone may be ambiguous. Combining it with additional markers helps distinguish vascular-associated cells from immune populations and supports more reliable interpretation of neuroimmune and tissue-mapping studies.
The same detectable membrane signal may arise from cells with different roles, including lymphatic endothelial cells and selected macrophages. Their location and accompanying marker profile therefore become important for assigning cellular identity. This distinction matters in nervous-system studies because vascular structures and immune populations can contribute differently to inflammation, drainage, and communication between neural tissues and the immune system.
Mapping Lyve-1 expression can show where lymphatic-associated structures and selected immune populations occur in relation to nervous-system tissues. In particular, it helps investigators examine meningeal lymphatic vessels, tissue immune organization, and pathways linking neural environments with immune activity. These spatial relationships provide context for studying brain fluid homeostasis, inflammation, and altered disease-associated states.
Researchers can detect the signal through antibody-based staining, immunohistochemistry, or flow cytometry. Staining and immunohistochemistry support localization within tissue, whereas flow cytometry supports analysis of labeled cell populations. The appropriate method depends on whether the study prioritizes spatial organization or population-level measurement, and interpretation remains dependent on additional markers.
A tissue-based approach, such as antibody staining or immunohistochemistry, is suited to determining where Lyve-1 positive cells or structures appear within a specimen. Flow cytometry instead examines labeled cells as a population. Pairing either approach with additional markers helps connect the observed signal to a cellular identity rather than treating every positive event as equivalent.
They are useful when investigators need to characterize meningeal lymphatic vessels, tissue immune populations, or neuroimmune communication. Analyses can support studies of lymphatic drainage, brain fluid homeostasis, inflammation, and changes associated with nervous-system disease. Because Lyve-1 is not exclusive to one cell type, these applications require marker-based interpretation to relate signal patterns to the biological process being examined.