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Isolation of Exosomes from the Plasma of HIV-1 Positive Individuals
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Exosomes from the Plasma of HIV-1 Positive Individuals

Isolation of Exosomes from the Plasma of HIV-1 Positive Individuals

DOI:
10.3791/53495-v

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06:46 min

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January 05, 2016

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, , , , , ,
1Department of Microbiology, Biochemistry, Immunology,Morehouse School of Medicine, 2Department of Medicine,Emory University School of Medicine, 3Department of Pathology and Laboratory Medicine,Emory University School of Medicine, 4Yerkes National Primate Research Center

Chapters

  • 00:05Title
  • 00:54Human Blood Processing
  • 02:20Purification of Exosomes
  • 03:41Acetylcholinesterase Activity Assay
  • 04:50Results: Characterization of Exosomes from HIV-1 Positive Human Plasma
  • 06:10Conclusion

Summary

Automatic Translation

Automatic Translation

Techniques describing a gradient procedure to separate exosomes from human immunodeficiency virus (HIV) particles are described. This procedure was used to isolate exosomes away from HIV particles in human plasma from HIV-infected individuals. The isolated exosomes were analyzed for cytokine/chemokine content.

Tags

Keywords: ExosomesHIV-1PlasmaVirus ParticlesUltracentrifugationIodixanol GradientVirus-infected CellsMicrovesiclesHIV InfectionCentrifugationPBS WashVirus Separation

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