Myeloid Cell Transplantation
Myeloid Cell Transplantation - Related Videos
18:48
In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients
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Cited by 1 •
2017
Here, we present a protocol to induce tolerance in transplantation, and assess in vitro and in vivo the suppressive capacity of distinct cell subsets from the recipient and the immune status of the recipient toward donor or exogenous antigens.
05:14
Studying Interactions between Myeloid Cells and CAR T Cells In Vitro and In Vivo
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2025
Given the limited xenograft models available to study interactions between human CART and myeloid cells, we established in vitro and in vivo models to understand the impacts of human macrophages on CART cells. Findings can potentially be generalized to evaluate macrophage roles in the tumor microenvironment and test macrophage-targeted immunotherapies.
06:39
Culture of myeloid dendritic cells from bone marrow precursors
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Cited by 22 •
2008
This video demonstrates the procedure for differentiating myeloid dendritic cells from mouse bone marrow. Isolation of mouse tibia and femur, and processing of bone marrow are demonstrated. Pictures demonstrating cell morphology before and after differentiation, and figures depicting cell phenotype and IL-12 production following maturation using CpG are shown.
09:07
Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus
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Cited by 27 •
2013
This protocol details a method to isolate antigen presenting cells from human thymus via different steps of enzymatic digestion of the tissue followed by density centrifugation of the single cell suspension and finally magnetic and/or FACS sorting of the cell populations of interest.
08:42
Transplantation of Whole Kidney Marrow in Adult Zebrafish
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Cited by 17 •
2007
In this article, we demonstrate a method to perform HCT in adult zebrafish.
FAQs
Conditioning or tissue injury can generate signals that improve the persistence and movement of transferred cells. These signals may help myeloid cells survive after administration, reach neural tissues, and establish themselves within injured regions. Their importance lies in linking the recipient’s physiological state to transplantation outcomes, allowing researchers to examine how the tissue environment shapes immune-cell behavior.
Cell trafficking across the blood-brain barrier is a central step. Signals associated with conditioning, injury, or local inflammation can promote movement from the circulation into neural tissues, where cells may then engraft. Studying this process helps distinguish whether an observed effect depends mainly on cell delivery, successful tissue entry, persistence, or interactions after arrival.
Local cytokines influence the activation and phenotype of myeloid cells after they enter neural tissues. In this context, phenotype refers to the functional characteristics the cells display in response to their surroundings. This environmental control matters because the same transferred population may behave differently in distinct neural regions or disease settings, affecting immune interactions and tissue responses.
A study generally requires selecting a myeloid-lineage population, administering it to a recipient, and examining whether conditioning or tissue injury supports survival, trafficking, and engraftment. Researchers then interpret how the transferred cells interact with neural tissues and respond to local cytokines. This workflow connects cell delivery with changes in immune activity, neural injury, or repair.
The approach is useful for investigating communication between immune cells, neurons, and glia during neuroinflammation, infection, and repair. It can also support disease modeling by placing defined myeloid populations into a neural context. These applications allow researchers to study how immune-cell behavior relates to damaged circuits and to evaluate whether transplanted cells alter those interactions.
Myeloid cell transplantation can provide a framework for testing engineered or therapeutic cells designed to deliver molecules, regulate inflammation, or promote recovery. Researchers can examine whether the cells reach neural tissues, engraft, and display the intended activity within the local cytokine environment. The resulting model links cellular engineering with functional questions about immune regulation and neural repair.