Neonatal Mice
Neonatal Mice - Related Videos
08:15
Measuring Left Ventricular Pressure in Late Embryonic and Neonatal Mice
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Cited by 18 •
2012
Measuring left ventricular pressure (LV) in embryonic and neonatal mice is described. Pressure is measured by inserting a needle connected to a fluid-filled transducer into the LV under ultrasound guidance. Care must be taken to maintain normal cardiac function during the experimental protocol.
03:45
Modeling Infection in Neonatal Mice Using Encapsulated Bioluminescent Bacteria
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2026
Source: Seman, B. G., et al. A Neonatal Imaging Model of Gram-Negative Bacterial Sepsis. J. Vis. Exp. (2020)The video demonstrates a neonatal mouse model for studying systemic infection using encapsulated bioluminescent bacteria. Neonatal pups are injected with high- or low-dose bacterial suspensions and returned to their mother for postnatal care. The encapsulated bacteria evade early immune detection, spread systemically, and are visualized over time using luminescence imaging. This model...
07:48
Myocardial Infarction in Neonatal Mice, A Model of Cardiac Regeneration
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Cited by 37 •
2016
This protocol describes a highly reproducible model of cardiac regeneration by surgical induction of myocardial infarction in the left ventricle of postnatal day 1 mice. The method involves induction of hypothermic anesthesia and ligation of the left anterior descending coronary artery.
08:02
Isolation of Pulmonary Artery Smooth Muscle Cells from Neonatal Mice
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Cited by 16 •
2013
We have developed a novel and reproducible technique to isolate primary cultures of pulmonary artery smooth muscle cells (PASMC) from mice as young as P7, thereby allowing better study of the signaling pathways involved in neonatal smooth muscle cell contraction and relaxation.
07:49
Spinal Cord Neurons Isolation and Culture from Neonatal Mice
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Cited by 8 •
2017
This study presents a technique for the isolation of neurons from WT neonatal mice. It requires the careful dissection of the spinal cord from the neonatal mouse, followed by the separation of neurons from the spinal cord tissue through mechanical and enzymatic cleavage.
FAQs
Developmental timing determines which neural processes are active and which outcomes can be observed. In neonatal mice, rapid changes in neuronal differentiation, migration, synapse formation, myelination, and sensory-circuit maturation create defined windows for examining age-dependent responses. Choosing a specific postnatal stage helps investigators relate an intervention or condition to the developmental process it affects.
Cellular and circuit changes are studied together because structural maturation can alter network function. As neurons differentiate and migrate, synapses form and myelination progresses, while sensory circuits also mature. These linked events provide a framework for connecting cellular mechanisms with circuit function, rather than treating early neural development as a single uniform process.
Age-dependent responses are useful because the neonatal nervous system changes quickly. A stimulus, injury, inflammatory condition, or intervention can produce different measurable effects depending on when it occurs during postnatal maturation. This temporal sensitivity allows researchers to ask whether an outcome reflects a general neural response or a process specific to an early developmental window.
Their sensory circuits mature during early postnatal life, so investigators can examine how circuit development changes the way neural networks process signals. This makes neonatal mice useful for relating developmental changes in circuit organization to measurable sensory responses and for identifying when altered processing emerges during nervous-system maturation.
A study begins by defining the postnatal developmental window, then selecting the level of analysis that matches the question: cellular mechanisms, circuit function, or behavior. Investigators can examine responses under controlled laboratory conditions and compare outcomes across developmental stages or experimental interventions. This organization links developmental timing with measurable neural or behavioral results.
Measurements can reveal changes in neuronal differentiation, migration, synapse formation, myelination, sensory-circuit maturation, circuit function, and behavior. The value lies in connecting these outcomes: cellular findings can be interpreted alongside network or behavioral changes. Such comparisons help determine how early developmental events relate to observed patterns of neural processing.
Neonatal mice can support studies of brain development, neurodevelopmental disorders, injury, inflammation, sensory processing, and behavior. Their rapid maturation and compatibility with controlled laboratory experiments allow investigators to examine these questions during defined developmental windows. The model can therefore connect cellular mechanisms with circuit function while also supporting evaluation of interventions during early life.