Ovulation Regulation
Ovulation Regulation - Related Videos
05:41
In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana
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Cited by 27 •
2017
This manuscript describes an in vitro ovule cultivation method that enables live-cell imaging of Arabidopsis zygotes and embryos. This method is utilized to visualize the intracellular dynamics during zygote polarization and the cell fate specification in developing embryos.
11:04
Unraveling the Role of Discrete Areas of the Rat Brain in the Regulation of Ovulation through Reversible Inactivation by Tetrodotoxin Microinjections
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2020
This protocol describes the construction of a low-cost microinjection system, its stereotaxic implantation into deep-brain structures and the procedure for timed microinjections of tetrodotoxin in awake and unrestrained rats. The goal is to reveal the participation of hypothalamic structures in the regulation of ovulation by inhibiting their neural activity.
03:20
Microinjection of Tetrodotoxin into the Brain of an Awake Female Rat to Inhibit Ovulation
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2025
Source: Silva, C. et al. Unraveling the Role of Discrete Areas of the Rat Brain in the Regulation of Ovulation through Reversible Inactivation by Tetrodotoxin Microinjections. J. Vis. Exp. (2020)This video demonstrates the protocol for microinjection of tetrodotoxin (TTX) into the hypothalamus of a freely moving rat to transiently inhibit neuronal activity, suppress gonadotropin-releasing hormone secretion, and prevent ovulation.
04:02
Embryo Transfer Into Rabbit: A Procedure for Laparoscopy-Guided Transfer of Embryos Into Oviduct of Ovulating Recipient Female Rabbit Model
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2025
This video describes the technique of transferring embryos into the oviduct of a recipient female rabbit via Laparoscopy. It allows embryo remodeling in rabbits during its migration through the oviduct, eventually helping in successful implantation. This technique can also be used for stored embryos with good efficiency.
02:14
GTPases and their Regulation
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2020
Guanine nucleotide-binding proteins (G-proteins), also known as GTPases, are a superfamily of proteins that regulate many cellular processes, such as cell signaling, vesicular transport, and the regulation of cell shape and motility. Mutation or dysfunction of these proteins can lead to disease. There are around 40,000 known G-proteins that can broadly be classified into two groups ‒ small G-proteins consisting of a single domain and large multi-domain G-proteins. Large G-proteins, also known...
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Pulsatile gonadotropin-releasing hormone, or GnRH, provides the hypothalamic signal that drives pituitary secretion of follicle-stimulating hormone and luteinizing hormone. This creates a communication sequence linking neural control to ovarian activity rather than treating follicle maturation as an isolated ovarian event. Changes that disturb hypothalamic signaling can therefore affect downstream pituitary output and ultimately impair ovulation.
Sustained high estradiol from the developing follicle switches the system from its earlier regulatory state to positive feedback. This change produces the mid-cycle luteinizing hormone surge, the endocrine event that triggers ovulation. The transition is important because follicle development alone is not sufficient; the timing and persistence of the ovarian estradiol signal determine whether the surge occurs.
The pituitary acts as the intermediary between hypothalamic GnRH pulses and the ovaries. In response to GnRH, it releases follicle-stimulating hormone and luteinizing hormone, and later generates the mid-cycle LH surge after sustained high estradiol feedback. This position allows pituitary signaling to coordinate follicle maturation with the final hormonal trigger for egg release.
Disruption at either the hypothalamic or pituitary level can interrupt the signaling chain required for ovulation. Inadequate hypothalamic input may fail to support appropriate gonadotropin secretion, while impaired pituitary signaling can prevent the ovarian response or the mid-cycle LH surge. Studying the location of the disruption helps connect abnormal reproductive function with specific neuroendocrine control points.
Ovulation regulation demonstrates how neural signals control an endocrine sequence involving the pituitary and ovaries. The hypothalamus supplies the initiating GnRH pattern, while hormonal feedback from the ovary modifies neuroendocrine output. This brain-to-gonad communication makes ovulation regulation a useful context for studying feedback control, signal timing, and interactions between nervous and reproductive physiology.
Mapping the hypothalamus-pituitary-ovary signaling pathway helps researchers investigate why ovulation fails and where regulation may be disrupted. The same framework supports research into fertility and menstrual disorders because it links follicle development, gonadotropin release, estradiol feedback, and the LH surge. These pathways are also relevant to hormonal contraception and to identifying potential diagnostic or treatment targets.