Neuroscience
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Unraveling the Role of Discrete Areas of the Rat Brain in the Regulation of Ovulation through Reversible Inactivation by Tetrodotoxin Microinjections
Chapters
Summary September 3rd, 2020
This protocol describes the construction of a low-cost microinjection system, its stereotaxic implantation into deep-brain structures and the procedure for timed microinjections of tetrodotoxin in awake and unrestrained rats. The goal is to reveal the participation of hypothalamic structures in the regulation of ovulation by inhibiting their neural activity.
Transcript
This protocol allows researchers to explore the nuclei of the neuronal circuits that regulates ovulation in female mammals at specific times of their estrous cycle.This technique can be adapted to study any process on the regulation of the brain in any species.Also they can less art fabricator in the lab lowering the overall cost.Demonstrating this procedure will be Montserrat Bola
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