Chirped-pulse Amplification
Chirped-pulse Amplification - Related Videos
11:09
Chromatin Isolation by RNA Purification (ChIRP)
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Cited by 380 •
2012
ChIRP is a novel and rapid technique to map genomic binding sites of long noncoding RNAs (lncRNAs). The method takes advantage of the specificity of anti-sense tiling oligonucleotides to allow the enumeration of lncRNA-bound genomic sites.
09:44
Rapid Amplification of cDNA Ends
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2023
Source: Pablo Sanchez Bosch2, Sean Corcoran2 and Katja Brückner1,2,3 1Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research 2Department of Cell and Tissue Biology, 3Cardiovascular Research Institute, University of California San Francisco, San Francisco, CA, USA Rapid Amplification of cDNA Ends (RACE) is a technique that allows amplification of full-length cDNA from mRNA by extending to the 3’ or 5’ end, even without prior knowledge of the sequence (Frohman et al.,...
09:30
A UV-Based ChIRP Method For The Verifiable Identification of Proteins That Directly Interact With LncRNA: From Cultured Cells To Mouse Testis
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2026
A UV-based ChIRP method to identify direct lncRNA-protein interactions.
02:41
Amplification of a Suicide Plasmid in a Bacterial Host
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2025
Source: Tomás, J. M., et al. Generation of Null Mutants to Elucidate the Role of Bacterial Glycosyltransferases in Bacterial Motility. J. Vis. Exp. (2022).This video demonstrates the initiator-dependent amplification of a suicide plasmid in E. coli. The plasmid, which carries an antibiotic resistance gene, replicates only in the presence of an initiator protein encoded by the host. After electroporation and incubation in recovery medium, the mixture is plated on antibiotic-containing agar.
01:16
Pulse
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2024
When the heart pumps blood out, arterial elastic fibers play a crucial role in sustaining a high-pressure gradient. They expand to accommodate the received blood and then recoil - a process known as the pulse that can be either manually palpated or electronically quantified. Despite a reduction in its effect with increased distance from the heart, elements of the pulse's systolic and diastolic components persist, observable even at the arteriole level. The pulse serves as a clinical indicator...
FAQs
Temporal stretching lowers a pulse’s peak power before it reaches the amplifier. Because amplification occurs while the pulse remains relatively low power, optical components experience less damaging intensity than they would during direct amplification of a compressed pulse. This enables later recompression to a short, intense pulse without exposing the amplifier to the full peak power.
Dispersive optics, including diffraction gratings, reverse the temporal spreading introduced during pulse stretching. They bring the different parts of the amplified pulse back into a brief time interval, restoring short duration and producing a much higher peak intensity. Their function is therefore central to converting a safely amplified pulse into one suitable for ultrafast biological applications.
High peak intensity allows substantial energy to be delivered within an extremely short interval, while the CPA sequence limits the risk of damaging the laser system itself. In bioengineering, this concentrated delivery supports confined energy deposition. The resulting spatial selectivity can improve precision during microsurgery and laser-based processing of biological materials.
The key characteristics are pulse duration, peak power, and the ability to control when high intensity occurs. Stretching and recompression separate amplification from the final intense-pulse interaction, while the brief recompressed duration supports localized energy delivery. Together, these features make the technique relevant when biological work requires strong optical effects with limited spatial spread.
A typical sequence begins by stretching the incoming pulse, followed by amplification while its peak power is reduced. Dispersive optics then recompress the amplified pulse to a brief duration. For bioengineering experiments, this sequence provides the intense ultrashort output needed for multiphoton microscopy, precision microsurgery, or processing biological materials.
In multiphoton microscopy, the intense ultrashort output supports imaging approaches that depend on concentrated optical energy. During precision microsurgery, confined deposition can improve spatial selectivity and help reduce collateral damage. These applications use the same controllable ultrafast behavior for different goals: resolving biological structures in imaging and modifying tissue or biological material with greater precision.
The very brief pulses provide a way to interrogate biological events on rapid timescales. By delivering controlled ultrafast light, researchers can examine fast biological dynamics and investigate how biological systems respond to tightly timed energy deposition. This capability also contributes to developing advanced imaging and therapeutic tools in bioengineering.