Journal
/
Biology
/
Chromatin Isolation by RNA Purification (ChIRP)
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Chromatin Isolation by RNA Purification (ChIRP)

Chromatin Isolation by RNA Purification (ChIRP)

DOI:
10.3791/3912-v

•

11:09 min

•

March 25, 2012

•

, ,
1Howard Hughes Medical Institute and Program in Epithelial Biology,Stanford University School of Medicine

Chapters

  • 00:05Title
  • 01:25Cross-linking Cells to Preserve RNA-Chromatin Interactions
  • 02:58Cell Lysis
  • 03:37Shearing DNA by Sonication
  • 05:13Chromatin Isolation by RNA Purification (ChIRP)
  • 08:43Results: Enrichment of Target Long Noncoding RNAs by ChIRP
  • 10:34Conclusion

Summary

Automatic Translation

Automatic Translation

ChIRP is a novel and rapid technique to map genomic binding sites of long noncoding RNAs (lncRNAs). The method takes advantage of the specificity of anti-sense tiling oligonucleotides to allow the enumeration of lncRNA-bound genomic sites.

Tags

Chromatin Isolation By RNA PurificationChIRPLong Noncoding RNAsChromatin StatesDosage CompensationImprintingDevelopmental Gene ExpressionLncRNAsChromatin Modification ComplexesPolycomb Repressive Complex 2Histone H3 Lysine 27 TrimethylationH3K27me3Gene-specific FashionCis RegulationTrans RegulationDrosophila LncRNAs RoX1RoX2X Chromosome Binding SitesHuman LncRNA HOTAIRPRC2 OccupancyGenome-wide EffectsModular ScaffoldsTERC RNATelomerase ComplexH3K4 Demethylase ComplexRNA Occupancy At Chromatin

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/3912?article_type=v

Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid
check_url/3912?article_type=v

Isolation and Purification of Drosophila Peripheral Neurons by Magnetic Bead Sorting
check_url/3912?article_type=v

Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides
check_url/3912?article_type=v

Isolation and Characterization of RNA-Containing Exosomes
check_url/3912?article_type=v

In vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion
check_url/3912?article_type=v

Isolation and Purification of Kinesin from Drosophila Embryos
check_url/3912?article_type=v

In vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection
check_url/3912?article_type=v

Rapid Isolation And Purification Of Mitochondria For Transplantation By Tissue Dissociation And Differential Filtration
check_url/3912?article_type=v

Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes
check_url/3912?article_type=v

RNA Isolation from Mouse Pancreas: A Ribonuclease-rich Tissue

Read Article