Fetal Fibroblasts
Fetal Fibroblasts - Related Videos
09:35
An Experimental System to Study Mechanotransduction in Fetal Lung Cells
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Cited by 7 •
2012
Mechanical forces play a key role in lung development and lung injury. Here, we describe a method to isolate rodent fetal lung type II epithelial cells and fibroblasts and to expose them to mechanical stimulation using an in vitro system.
08:04
Murine Fetal Echocardiography
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Cited by 7 •
2013
Fetal and perinatal death is a common feature when studying genetic alterations affecting cardiac development. High-frequency ultrasound imaging has improved 2-D resolution and can provide excellent information on early cardiac development and is an ideal method to detect the impact on cardiac structure and function prior to death.
09:43
Isolation, Culture, and Characterization of Prostate Cancer-Associated Fibroblasts
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Cited by 1 •
2025
This protocol provides a comprehensive procedure to isolate, expand, and immortalize fibroblasts from radical prostatectomies. Moreover, it describes assays developed to assess the functional effects of fibroblast-tumor cell crosstalk, taking advantage of both treatment with conditioned medium and co-culturing.
15:43
Experimental Generation of Carcinoma-Associated Fibroblasts (CAFs) from Human Mammary Fibroblasts
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Cited by 25 •
2011
Carcinoma-associated fibroblasts (CAFs) rich in myofibroblasts present within the tumour stroma, play a major role in driving tumour progression. We developed a coimplantation tumour xengraft model for experimentally generating CAFs from human mammary fibroblasts. The protocol describes how to establish CAF myofibroblasts that acquire an ability to promote tumourigenesis.
14:19
Fetal Echocardiography and Pulsed-wave Doppler Ultrasound in a Rabbit Model of Intrauterine Growth Restriction
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Cited by 11 •
2013
We describe examination of fetal cardiac function with contemporary functional fetal echocardiography and fetoplacental Doppler ultrasound using the VisualSonics VEVO 2100 microultrasound in a surgically induced model of intrauterine fetal growth restriction in a rabbit.
FAQs
Developmental signals can alter fetal fibroblast gene expression, growth, migration, and extracellular matrix organization. These responses connect the cells’ developmental state with tissue formation because matrix production and arrangement influence how developing tissues are supported. In culture, examining coordinated changes helps researchers distinguish regulated developmental behavior from effects associated with a genetic perturbation.
Extracellular matrix production provides a way to examine how fetal fibroblasts contribute to tissue organization. Structural proteins such as collagen reveal changes in matrix synthesis, while the arrangement of that matrix reflects cellular responses to developmental signals. Measuring these features can therefore connect gene regulation with physical changes relevant to developing tissues.
Their developmental origin gives fetal fibroblasts a cellular state suited to studying tissue differentiation and development, whereas adult cells may reflect a later stage of tissue biology. Results from fetal cultures may therefore not match findings in adult cells. Researchers must interpret differences in gene expression, growth, or matrix behavior in light of this developmental context.
A study can maintain fetal fibroblasts in culture, observe their proliferation, and examine gene expression or extracellular matrix production. Researchers may then introduce an experimental perturbation and compare cellular responses with an appropriate unperturbed condition. Depending on the research question, the analysis can also examine chromosome abnormalities, mutations, growth, migration, or matrix organization.
Cultured fetal fibroblasts can support analyses of gene expression, chromosome abnormalities, and mutations within a living cellular system. Because the cells proliferate in culture, investigators can examine these features alongside growth and responses to experimental perturbations. This combination helps connect genetic findings with cellular behavior rather than evaluating genetic information in isolation.
They are useful when researchers need to examine how an inherited genetic change relates to cellular behavior during development. Cultures can provide a setting for analyzing gene expression, growth, matrix production, and responses to experimental perturbations. Their developmental origin also supports investigation of disorders involving tissue differentiation, while comparisons with adult cells help define the limits of interpretation.
Fetal fibroblast cultures can be used to explore how experimental perturbations affect developmental cellular processes and genetic disease-related phenotypes. Observing changes in gene expression, proliferation, migration, or matrix organization may help evaluate biological responses relevant to therapeutic strategies. These findings remain context-dependent because cultured fetal cells may behave differently from adult cells or other tissues.