Bronchial Tissue Explants
Bronchial Tissue Explants - Related Videos
14:32
Primary Human Bronchial Epithelial Cells Grown from Explants
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Cited by 19 •
2010
Here we describe a detailed method for growing primary human bronchial epithelial cells from explants of human bronchial airway tissue including differentiated growth on an air-liquid interface. This method provides an abundant source of primary cells for investigating the role of the airway epithelium in human lung health and disease.
07:45
Explant Culture of Neural Tissue
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2023
The intricate structure of the vertebrate nervous system arises from a complex series of events involving cell differentiation, cell migration, and changes in cell morphology. Studying these processes is essential to our understanding of nervous system function as well as our ability to diagnose and treat disorders that result from abnormal development. However, neural tissues are relatively inaccessible for experimental manipulations, especially in embryonic mammals. As a result, many...
09:50
Impact Assessment of Repeated Exposure of Organotypic 3D Bronchial and Nasal Tissue Culture Models to Whole Cigarette Smoke
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Cited by 41 •
2015
The aim of this protocol is to expose human organotypic 3D bronchial and nasal tissue models to mainstream cigarette smoke (CS) at the air-liquid interface. The impact of CS on the tissues is then investigated using a cytochrome P450 activity assay, a cilia beating measurement, and a systems biology approach.
14:39
Bronchial Thermoplasty: A Novel Therapeutic Approach to Severe Asthma
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Cited by 4 •
2010
Bronchial thermoplasty is a non-drug procedure for severe persistent asthma that delivers thermal energy to the airway wall in a precisely controlled manner to reduce excessive airway smooth muscle. Reducing airway smooth muscle decreases the ability of the airways to constrict, thereby reducing the frequency of asthma attacks.
10:57
Using In Vivo and Tissue and Cell Explant Approaches to Study the Morphogenesis and Pathogenesis of the Embryonic and Perinatal Aorta
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2017
Protocols for studying the embryonic and perinatal murine aorta using in vivo clonal analysis and fate mapping, aortic explants, and isolated smooth muscle cells are detailed here. These diverse approaches facilitate the investigation of the morphogenesis of the embryonic and perinatal aorta in normal development and the pathogenesis in disease.
FAQs
Maintaining epithelial, stromal, and immune-cell relationships allows responses to arise within a tissue context rather than from isolated cell populations. These interactions can influence epithelial integrity, cytokine production, immune-cell behavior, and pathogen responses. Consequently, researchers can examine coordinated airway reactions and identify effects that may be difficult to interpret in simplified culture systems.
The model retains aspects of native airway organization while allowing controlled exposure to microbial agents, inflammatory stimuli, or candidate treatments outside the body. This combination provides more physiological context than isolated cells and greater experimental control than whole-organism studies. Findings can therefore support mechanistic analysis and inform decisions about progression to more complex models.
Researchers can monitor epithelial integrity, cytokine production, immune-cell behavior, and pathogen responses as complementary indicators. Epithelial changes describe tissue condition, cytokines indicate inflammatory signaling, immune-cell behavior reflects cellular responses, and pathogen measurements address host-microbe interactions. Examining these outcomes together helps connect a stimulus with both tissue damage or preservation and immune activity.
A study generally maintains the tissue outside the body, exposes it to a microbial agent, inflammatory stimulus, or candidate treatment, and then monitors relevant biological outcomes. The selected observations may include epithelial integrity, cytokine production, immune-cell behavior, and pathogen responses. This workflow enables direct comparison of tissue reactions under defined experimental conditions.
They are particularly useful when a question concerns airway inflammation, host-pathogen interactions, or treatment effects that depend on communication among epithelial, stromal, and immune cells. The system supports mechanistic studies while preserving tissue-level context. It can also help evaluate whether observations from simpler experiments warrant testing in more complex models.
Candidate treatments can be applied while researchers monitor changes in epithelial integrity, cytokine production, immune-cell behavior, and pathogen responses. These measurements can indicate whether an intervention modifies inflammation, preserves tissue condition, or affects pathogen-related outcomes within an organized airway sample. The resulting evidence may guide further investigation before progression to whole-organism studies.