Automated Cell Culture

Automated cell culture is the use of robotic instruments, software, and controlled laboratory equipment to grow and maintain cells with limited manual intervention. These systems coordinate tasks such as media dispensing, cell seeding, incubation, feeding, passaging, and imaging under defined temperature, gas, and timing conditions, while standardized workflows improve consistency between cultures. In medicine, automated cell culture supports drug screening, disease modeling, toxicity testing, and the production of cells for research and therapeutic development. By increasing throughput, reducing handling variability, and enabling continuous monitoring, automation helps researchers generate more reproducible data and scale complex cell-based studies.

Automated Cell Culture - Related Videos

Research

JoVE Journal - Immunology and Infection

Automated Cell Enrichment of Cytomegalovirus-specific T cells for Clinical Applications using the Cytokine-capture System

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Cited by 23 •

2015

The goal of this protocol is to manufacture pathogen-specific clinical-grade T cells using a bench-top, automated, second generation cell enrichment device that incorporates a closed cytokine capture system and does not require dedicated staff or use of a GMP facility. The cytomegalovirus pp65-specific-T cells generated can be directly administered to patients.

An Automated Culture System for Maintaining and Differentiating Human-Induced Pluripotent Stem Cells

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2024

Here, we present a protocol for an automated cell culture system. This automated culture system reduces labor and benefits the users, including researchers unfamiliar with handling induced pluripotent stem (iPS) cells, from the maintenance of iPS cells to differentiation into various types of cells.

Research

JoVE Journal - Biochemistry
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Semi-Automated Phenotypic Analysis of Functional 3D Spheroid Cell Cultures

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Cited by 2 •

2023

We present a protocol for growing high-reproducible spheroids and their phenotypic characterization using image capture and proteomics.

Research

JoVE Journal - Biology
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Determining Cell Number During Cell Culture using the Scepter Cell Counter

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Cited by 56 •

2010

The Scepter Cell Counter is a handheld automated device that can be used to count cells, monitor cell diameter and volume, and be used to check the health and quality of cellular populations from one culture to the next.

Image-Guided Robotic Cell Microinjection: An Automated High-Throughput Technique for the Precise Delivery of Fluorescently-Labeled Polysaccharides Into Single Neuronal Cells in Organotypic Brain Slice Cultures

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2025

In this video, we demonstrate the robotic microinjection of fluorescently-labeled polysaccharides into pyramidal neurons in organotypic slices of mouse embryonic brain using an autoinjector. The autoinjector is a robotic platform that utilizes images acquired from a microscope for the precise delivery of biomolecules of interest into single cells.

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