Glioblastoma Resection
Glioblastoma Resection - Related Videos
05:45
Quantitative Immunohistochemistry of the Cellular Microenvironment in Patient Glioblastoma Resections
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Cited by 10 •
2017
This protocol was developed to quantitatively identify tumor microenvironment components in glioblastoma patient resections using chromogenic immunohistochemistry and ImageJ.
04:46
Glioblastoma Relapse Post-Resection Model for Therapeutic Hydrogel Investigations
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Cited by 4 •
2023
The present protocol establishes a glioblastoma (GBM) relapse post-resection model using microscopy to investigate the therapeutic effect of an injectable, bioresponsive hydrogel in vivo.
03:58
Brain Tumor Resection in a Mouse Using a Minimally Invasive Resection System
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2025
Source: Alomari, S., et al. Implementation of Minimally Invasive Brain Tumor Resection in Rodents for High Viability Tissue Collection. J. Vis. Exp. (2022)This video demonstrates the use of a minimally invasive resection system (MIRS) to excise tumor tissue from an anesthetized mouse implanted with a tumor. The procedure involves securing the mouse onto a stereotactic frame, reopening the previous surgical site to access the brain, and inserting a specialized cannula that utilizes suction and a...
02:36
Generating a Glioblastoma Rat Model
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2025
Source: Bolcaen, J., et. al., PET and MRI Guided Irradiation of a Glioblastoma Rat Model Using a Micro-irradiator. J. Vis. Exp. (2017)This video demonstrates the procedure for establishing a glioblastoma rat model by stereotactically injecting brain tumor-causing glioblastoma cells into the prefrontal cortex.
09:24
Isolation and Expansion of Human Glioblastoma Multiforme Tumor Cells Using the Neurosphere Assay
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Cited by 66 •
2011
This video protocol demonstrates the isolation and expansion of stem like cells from surgically resected human glioblastoma mutliforme (GBM) tumor tissue using the neurosphere assay culture method.
FAQs
Complete removal is often limited by glioblastoma cells that extend into nearby brain tissue beyond the visible tumor. Removing every infiltrating cell could threaten critical neural regions, so the surgical goal is calibrated to safety rather than tumor clearance alone. This balance explains why maximal safe resection supports local disease control without implying that microscopic infiltration has been eliminated.
Preoperative imaging helps define the lesion and its relationship to surrounding structures, while intraoperative navigation guides the surgeon during the operation. Functional mapping identifies brain regions important for function, allowing the team to adjust the resection boundary when tumor lies near critical tissue. Together, these tools support maximal removal within neurologic safety limits.
Tissue sampling serves two linked purposes: it confirms the diagnosis through histopathological examination and supplies material for molecular characterization. These findings establish the biological identity of the lesion and can inform subsequent management after surgery. Thus, resection provides diagnostic value in addition to its surgical role.
Planning begins with preoperative imaging, which helps the surgical team assess the tumor and nearby brain regions. During resection, intraoperative navigation provides positional guidance, and functional mapping helps identify critical areas. Surgeons then remove tumor tissue while preserving these regions as far as safely possible. Tissue is sampled for histopathological and molecular analysis.
By reducing tumor burden, surgery may relieve symptoms and create a foundation for subsequent care. The amount removed also matters for local disease control, although infiltrating cells mean that surgery alone is not curative. Radiotherapy and chemotherapy are used in combination with the operation as subsequent treatment.
The procedure connects neurosurgery, pathology, imaging, and oncology. Neurosurgical decisions determine how much tumor can be removed safely; pathology and molecular testing characterize the sampled tissue; and the resulting information supports subsequent treatment with radiotherapy and chemotherapy. This multidisciplinary connection makes resection both a treatment step and a source of clinically relevant evidence.