Ivis Imaging System
Ivis Imaging System - Related Videos
08:53
In vivo Bioluminescent Imaging of Mammary Tumors Using IVIS Spectrum
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Cited by 58 •
2009
Mammary tumor cells expressing luciferase are implanted subcutaneously in mice and visualized using optical imaging to monitor tumor growth and development non-invasively in a longitudinal study.
10:21
In Vivo Imaging Systems (IVIS) Detection of a Neuro-Invasive Encephalitic Virus
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Cited by 11 •
2012
Utilizing luciferase and in vivo imaging systems (IVIS) as a novel means to identify disease endpoints before clinical developments occur. IVIS has allowed us to visualize in real time the invasion of encephalitic viruses over multiple days, providing a more accurate disease model for future study. It has also allowed us to identify the potential protective features of antivirals and vaccines faster than currently utilized animal models. The capability to utilize individual animals over...
09:53
In vivo Imaging of Transgenic Leishmania Parasites in a Live Host
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Cited by 65 •
2010
An in vivo imaging system is used to generate quantitative measurements of murine infection with the Trypanosomatid protozoan Leishmania. This is a non-invasive and non-lethal method for detecting parasites expressing luciferase within many tissues throughout the course of chronic Leishmania spp. infection.
10:23
Using Luciferase to Image Bacterial Infections in Mice
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Cited by 22 •
2011
Methods for bioluminescence imaging of bacterial infections in living animals are decribed. Pathogens are modified to express luciferase allowing optical whole body imaging of infections in live animals. Animal models can be infected with luciferase expressing pathogens and the resulting course of disease visualized in real-time by bioluminescence imaging.
11:43
Advanced Animal Model of Colorectal Metastasis in Liver: Imaging Techniques and Properties of Metastatic Clones
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Cited by 6 •
2016
The ability of metastatic clones to colonize distant sites depends on their proliferation capacity and/or their ability to survive in the host microenvironment without significant proliferation. Here, we present an animal model that allows quantitative visualization of both types of liver colonization by metastatic clones.
FAQs
The two signal modes rely on different light-generation steps. Bioluminescence requires luciferase-expressing cells or microbes and a substrate, whereas fluorescence requires an excitation step that causes labeled targets to emit light. This distinction determines what must be present in the biological model and how the signal is produced before the sensitive camera records it.
Each component supports a different stage of signal detection. The substrate enables luciferase-expressing cells or microbes to produce bioluminescent light, while excitation initiates emission from fluorescently labeled targets. The sensitive camera then captures the emitted light and maps its distribution, linking the optical signal to its location in the living organism.
Repeated, noninvasive measurements allow researchers to follow changes in the same living organism across multiple time points. This longitudinal approach can show how biological activity develops, declines, or responds during a study, while reducing the need to sacrifice animals at every observation point. The resulting time course adds context that a single measurement cannot provide.
A study begins with a model containing either luciferase-expressing cells or microbes, or fluorescently labeled targets. For bioluminescence, the relevant substrate is provided; for fluorescence, the target is exposed to excitation. The system’s sensitive camera captures the resulting light and maps its distribution, producing an image for tracking biological activity.
In preclinical medicine, IVIS imaging can be applied to tumor growth, infection, gene expression, drug delivery, and treatment response studies. Its value differs by model: signals can help track disease-associated activity, labeled biological targets, or changes occurring after an intervention. Imaging over time also supports comparisons across stages of the same experiment.
By imaging living organisms repeatedly, researchers can observe biological changes during treatment rather than relying only on an endpoint measurement. In studies of tumors, infections, drug delivery, or gene expression, the mapped optical signal can be followed across time to assess changing activity and treatment response while limiting animal sacrifice at each time point.