Retinal Bleb Formation
Retinal Bleb Formation - Related Videos
01:16
Cell Motility through Blebbing
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2023
Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases. Blebbing Through the Matrix In multicellular...
04:13
Subretinal Injection of Viral Vectors into the Retinal Pigment Epithelium in a Mouse Model
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2025
Source: Park, S. W., et al. Limbal Approach-Subretinal Injection of Viral Vectors for Gene Therapy in Mice Retinal Pigment Epithelium. J. Vis. Exp. (2015)The video describes the method for subretinal injection of viral vectors into the retinal pigment epithelium (RPE) in a mouse model. It details the procedure from anesthesia and eye preparation to precise lentiviral vector delivery, ensuring gene integration and expression in the RPE.
10:10
Ultrahigh Resolution Mouse Optical Coherence Tomography to Aid Intraocular Injection in Retinal Gene Therapy Research
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Cited by 6 •
2018
Here we demonstrate a novel approach to using high resolution spectral-domain optical coherence tomography (HR-SD-OCT) to assist delivery of gene therapy agents into the subretinal space, assess its areal coverage, and characterize photoreceptor vitality.
02:54
Preparation of Mouse Retinal Cryosections to Assess Retinal Permeability to Adeno-Associated Virus
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2026
Source: Vacca, O., et al. Using Adeno-associated Virus as a Tool to Study Retinal Barriers in Disease. J. Vis. Exp. (2015)This video demonstrates the step-by-step procedure for preparing mouse retinal cryosections to visualize adeno-associated virus permeability across disrupted retinal barriers.
09:17
Using Retinal Imaging to Study Dementia
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Cited by 19 •
2017
The retina shares prominent similarities with the brain and thus represents a unique window to study vasculature and neuronal structure in the brain non-invasively. This protocol describes a method to study dementia using retinal imaging techniques. This method can potentially aid in diagnosis and risk assessment of dementia.
FAQs
These three features determine where and how long an injected material contacts the subretinal space. Bleb size influences the area reached, location affects which retinal region receives the substance, and duration affects the time available for exposure or delivery. Excessive or poorly controlled values may increase the likelihood of tissue damage, making precise control important in retinal research.
The injected volume must be controlled because it directly influences the extent of tissue lifting and the resulting bleb dimensions. A smaller or larger separation changes the distribution of a balanced solution, viral vector, cell preparation, drug, or other test substance. Volume control therefore helps researchers manage delivery coverage while limiting unnecessary retinal stress.
Placement beneath the retina positions the delivered material next to the retinal tissues being studied or treated. This location supports several experimental goals, including gene delivery, cell transplantation, and drug administration. It also means that the injection site and the resulting bleb location must be considered when interpreting distribution, treatment effects, or possible tissue injury.
For delivery studies, the bleb provides a temporary space for introducing a viral vector, cells, drug, balanced solution, or another test substance beneath the retina. In experimental injury modeling, the formation process itself helps model retinal disturbance. The same controlled separation can therefore support either therapeutic investigation or study of retinal damage, depending on the research objective.
The procedure uses a controlled injection of a selected material beneath the retina, followed by gentle lifting of the tissue as fluid accumulates in the subretinal space. Researchers monitor the resulting bleb as a localized, temporary separation. Control of the injected volume, location, and persistence is central to achieving a useful experimental condition without excessive tissue disruption.
The source material identifies balanced solution, viral vectors, and other test substances as possible injected materials. The technique also supports delivery of drugs and cell preparations for research applications. Material choice depends on the experimental purpose, such as evaluating gene delivery, transplantation, administration of a treatment, or creating a controlled model for investigating retinal injury.
Researchers use this approach when they need to place material into the subretinal space for a focused retinal investigation. Supported applications include subretinal gene delivery, cell transplantation, drug administration, and experimental retinal injury modeling. Because bleb characteristics affect distribution and tissue response, the method also helps evaluate how a treatment or test substance behaves in a defined retinal setting.