Calcium-sensitive Proteins
Calcium-sensitive Proteins - Related Videos
03:22
Calcium-Dependent Hydrophobic Interaction Chromatography: A Technique to Purify Calcium-Binding Proteins Based on Hydrophobic Interactions
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2025
In this video, we demonstrate the purification of calcium-binding protein from a dialyzed cell lysate through calcium-dependent hydrophobic interaction chromatography. The calcium-binding proteins expose a hydrophobic region upon binding with calcium, facilitating interaction with a hydrophobic group on resin. Later these proteins are eluted using calcium chelator EDTA that reverses the interaction.
11:49
Characterization of G Protein-coupled Receptors by a Fluorescence-based Calcium Mobilization Assay
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Cited by 26 •
2014
The here described fluorescence-based calcium mobilization assay is a medium-throughput reverse pharmacology screening system for the identification of functionally activating ligand(s) of orphan G protein-coupled receptors (GPCRs).
10:57
Fast and Sensitive Colloidal Coomassie G-250 Staining for Proteins in Polyacrylamide Gels
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Cited by 328 •
2009
This video shall popularize a colloidal Coomassie G-250 staining protocol according to Kang et al. for the detection of average 4 ng protein in gels. The staining is completed within 2 hours and without any effort. We routinely use Kang's protocol for analytical purposes in gel-based proteomics.
04:46
Calcium Influx Assay to Measure Mitochondrial Calcium Uptake in Cultured Cells
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2025
This video describes a confocal microscopy imaging-based assay to measure mitochondrial calcium uptake in cultured cells. The assay uses the calcium-sensitive fluorescent dye Rhod-2/AM to measure the mitochondrial calcium in permeabilized cultured cells.
10:09
Detection of Detergent-sensitive Interactions Between Membrane Proteins
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Cited by 2 •
2018
We describe a protocol for detection of detergent-sensitive interactions between membrane proteins using binding of the sorting receptor, sortilin, to the first luminal loop of the glucose transporter protein, GLUT4, as an example.
FAQs
When Ca2+ binds an EF-hand motif, the protein can undergo a conformational change that modifies its molecular behavior. This structural shift may strengthen or weaken interactions with other proteins, alter enzyme activity, influence ion-channel behavior, or redirect the protein within the cell. These changes allow fluctuations in calcium concentration to produce coordinated neuronal responses.
A calcium-sensitive protein can produce different effects depending on where it is positioned within a neuron. Calcium-dependent changes in localization may bring the protein into contact with particular enzymes, ion channels, or signaling partners. This spatial organization helps connect local calcium signals with specific outcomes, including changes in excitability, neurotransmitter release, or gene expression.
Their effects extend beyond a single signaling step because calcium-dependent conformational changes can regulate multiple molecular targets. In neurons, this provides a route from activity-related calcium changes to neurotransmitter release, synaptic plasticity, altered excitability, and gene expression. The particular outcome depends on which calcium-sensitive proteins and downstream partners are present in the relevant cellular compartment.
Their ability to respond to calcium provides the molecular basis for tools that monitor neural signaling through calcium changes. Genetically encoded calcium indicators use this calcium-sensitive behavior to produce a measurable signal linked to neuronal activity. Such tools help researchers track signaling dynamics and examine how activity develops across neurons or neural circuits.
These proteins connect calcium dynamics with several major areas of neural function. Studying them can clarify how neurons release neurotransmitters, modify synaptic strength, regulate excitability, and alter gene expression. The same framework also supports investigation of neural circuits, because calcium-sensitive indicators can help monitor signaling associated with neuronal activity.
Disrupted calcium sensing can interfere with the molecular pathways that normally coordinate neuronal activity and communication. Because calcium-sensitive proteins participate in neurotransmitter release, synaptic plasticity, excitability, and gene expression, abnormal regulation may contribute to impaired circuit function. Their study therefore provides a way to connect altered calcium signaling with mechanisms associated with neurodegenerative disease.