Hippocampal Astrocytes
Hippocampal Astrocytes - Related Videos
08:32
Sex Differences in Mouse Hippocampal Astrocytes after In-Vitro Ischemia
0 Views •
Cited by 9 •
2016
Astrocytes are one of the most important key players in the central nervous system (CNS). Here, we are reporting a practical method of sexed hippocampal astrocyte culture protocol in order to study the mechanisms underlying the astrocyte function in male and female neonate pups after in-vitro ischemia.
02:19
Two-Photon Microscopy of Astrocytes and Excitatory Neurons in a Cleared Mouse Hippocampal Tissue
0 Views •
2025
Source: Refaeli, R., et al., Investigation of Spatial Interaction Between Astrocytes and Neurons in Cleared Brains. J. Vis. Exp. (2022) The video demonstrates the preparation of a chamber and the neuroimaging of cleared mouse hippocampal tissue using two-photon microscopy to visualize the spatial interactions between fluorescently labeled astrocytes and excitatory neurons.
12:48
Measuring Near Plasma Membrane and Global Intracellular Calcium Dynamics in Astrocytes
0 Views •
Cited by 16 •
2009
We describe how to measure near membrane and global intracellular calcium dynamics in cultured astrocytes using total internal reflection and epifluorescence microscopy.
12:47
Inducing Plasticity of Astrocytic Receptors by Manipulation of Neuronal Firing Rates
0 Views •
Cited by 8 •
2014
Here we describe an adaptation of protocols used to induce homeostatic plasticity in neurons for the study of plasticity of astrocytic G protein-coupled receptors. Recently used to examine changes in astrocytic group I mGluRs in juvenile mice, the method can be applied to measure scaling of various astrocytic GPCRs, in tissue from adult mice in situ and in vivo, and to gain a better appreciation of the sensitivity of astrocytic receptors to changes in neuronal activity.
06:41
Astrocyte Isolation: A Method to Obtain Pure Preparation of Mouse Cortical Astrocytes
0 Views •
2023
In this video, we describe the method to obtain a pure preparation of astrocytes from newly born mice.
FAQs
Their processes surround synapses and help control extracellular ions and neurotransmitters. This regulation limits unwanted changes in the local environment while allowing neural signals to be transmitted appropriately. Because synaptic communication depends on stable chemical conditions, these astrocytic functions can affect how hippocampal circuits process information and maintain network stability.
Neural activity can trigger astrocytic responses that modulate synaptic signaling rather than merely support it. This creates a two-way relationship in which active circuits influence astrocytes, while astrocytes alter the local signaling environment. Such interactions are relevant to synaptic plasticity, the capacity of circuits to change, and the information-processing functions of the hippocampus.
Astrocytes provide metabolic support while their processes interact with both synapses and blood vessels. This positioning links local neural activity with the conditions needed for hippocampal circuit operation. Studying that relationship helps researchers consider how support functions and signaling regulation work together to influence plasticity and preserve stable network activity.
Research can examine how astrocyte interactions with hippocampal circuits influence information processing and plasticity, two processes closely related to learning and memory. This perspective expands investigation beyond neuron-to-neuron communication by considering glial contributions to circuit behavior. It can help clarify how cellular interactions within the hippocampus support memory formation.
Their contribution to local chemical regulation, metabolic support, and synaptic signaling makes them relevant to studies of how hippocampal circuits develop and function. Investigating these roles can help clarify neurodevelopmental mechanisms involving information processing and network stability. The same framework may also show how altered glial support affects cognitive function over time.
If astrocytic regulation of ions, neurotransmitters, metabolic support, or activity-dependent signaling is disrupted, hippocampal circuits may not maintain their usual balance. Research on this possibility helps connect glial dysfunction with cognitive impairment and epileptic activity. The hippocampus is therefore a useful setting for examining how altered neuron-glia interactions affect network behavior and memory-related functions.