Neuron Macrophage Coculture
Neuron Macrophage Coculture - Related Videos
05:44
Developing a Neuron and Macrophage Coculture In Vitro
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2025
This video shows the co-culturing of murine dorsal root ganglion neurons and macrophages. Neurons are seeded on a coated plate, while macrophages are collected from an euthanized mouse, treated, and added to the culture insert. Treatment with db-cAMP activates neurons and prompts macrophages to adopt a pro-regenerative phenotype to support neuronal outgrowth.
09:23
Coculture Assays to Study Macrophage and Microglia Stimulation of Glioblastoma Invasion
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Cited by 19 •
2016
Understanding the malignant behavior of cancer requires creating accurate models of how tumor cells interact with components of the tumor microenvironment, such as macrophages. Here we describe two methods to study glioblastoma cell interaction with tumor associated macrophages and microglia where the effect on glioblastoma invasion is assessed.
11:08
The Indirect Neuron-astrocyte Coculture Assay: An In Vitro Set-up for the Detailed Investigation of Neuron-glia Interactions
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Cited by 26 •
2016
This protocol describes the indirect neuron-astrocyte coculture for compartmentalized analysis of neuron-glia interactions.
03:24
Coculturing of Retinal Ganglion Neurons with Olfactory Ensheathing Glia
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2025
This video demonstrates the procedure to isolate retinal ganglion neurons (RGNs) from a rat's retina and coculture them with olfactory ensheathing glia (OEG) cells. This method allows for studying the neuro-regenerative potential of OEGs after neural injury.
03:32
Using a Macrophage Conditioned Medium to Promote Neuron Extensions
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2025
This video demonstrates an assay for promoting the growth of neuron projections known as neurite outgrowth using the macrophage-conditioned medium. Co-culture neurons and macrophages with cAMP and harvest the macrophage-derived conditioned medium. Growing the neurons with the conditioned medium promotes neurite outgrowths. These outgrowths are fluorescently labeled and visualized using a fluorescence microscope.
FAQs
Macrophages respond to signals from neurons as well as to environmental cues in the culture. These inputs can shift their activation state, which changes the immune signals they produce. Studying this response helps researchers connect neuronal communication with immune regulation and examine how macrophage behavior may contribute to inflammation, injury, or repair in nervous-system models.
The two arrangements help separate contact-dependent effects from effects caused by soluble factors. Direct contact allows cells to influence one another through physical interaction and released signals, whereas compartmentalized cultures test communication without direct contact. This comparison can clarify whether a neuronal or macrophage response requires cell contact or results from molecules released into the surrounding environment.
Macrophages can release cytokines, growth factors, and inflammatory mediators that influence neuronal responses. Depending on the experimental context, these signals may be examined in relation to neuronal survival, injury, repair, or function. Measuring neuronal outcomes alongside macrophage activation provides a way to study how immune modulation shapes neural processes in a controlled in vitro setting.
A basic workflow establishes neurons and macrophages in the same experimental system, then selects either direct contact or a compartmentalized arrangement. Researchers introduce the environmental conditions being studied and observe changes in both cell populations. The design should preserve the distinction between contact-mediated and soluble-factor-mediated communication so the resulting neuronal and immune effects can be interpreted.
This model is useful when the research question concerns communication between neural and immune cells rather than isolated cell behavior. It supports investigations of neuroinflammation, neuronal injury and repair, neurodegenerative disease mechanisms, and nervous-system immune responses. Including both populations allows researchers to examine how changing macrophage activity influences neuronal survival and function.
The system can reveal changes in macrophage activation state together with effects on neuronal survival and function. It also helps indicate whether those effects depend on direct cell interaction or on soluble mediators. These outcomes provide mechanistic context for studying neuroinflammation and for testing how immune modulation may alter neuronal injury, repair, or disease-related processes.