Optical Reflectance Imaging
Optical Reflectance Imaging - Related Videos
08:30
Imaging Odor-Evoked Activities in the Mouse Olfactory Bulb using Optical Reflectance and Autofluorescence Signals
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Cited by 9 •
2011
This article presents the protocols of intrinsic optical signals and flavoproteins autofluorescence signals imaging to map odor-evoked activities at the surface of the olfactory bulb in mice.
13:59
Reflection and Refraction
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2023
Source: Derek Wilson, Asantha Cooray, PhD, Department of Physics & Astronomy, School of Physical Sciences, University of California, Irvine, CA Light travels at different speeds depending on the material through which it is propagating. When light travels from one material to another, it will either slow down or speed up. In order to conserve energy and momentum, the light must change the direction in which it propagates. This bending of light is known as refraction. Some fraction of the...
02:51
Cell Imaging Using Total Internal Reflection Fluorescence Microscopy
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2025
Source: Daniele, F., et. al TIRFM and pH-sensitive GFP-probes to Evaluate Neurotransmitter Vesicle Dynamics in SH-SY5Y Neuroblastoma Cells: Cell Imaging and Data Analysis. J. Vis. Exp. (2015).This video demonstrates the imaging of human neuroblastoma cells using a total internal reflection fluorescence (TIRF) microscope to visualize fluorophore-tagged synaptic vesicles. The cells are first focused in epifluorescence mode. Adjustments are then made to achieve total internal reflection,...
11:04
Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor (IRIS)
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Cited by 2 •
2011
Quantitative, high-throughput, real-time, and label-free biomolecular detection (DNA, protein, etc.) on SiO2 surfaces can be achieved using a simple interferometric technique which relies on LED illumination, minimal optical components, and a camera. The Interferometric Reflectance Imaging Sensor (IRIS) is inexpensive, simple to use, and amenable to microarray formats.
08:31
Three-dimensional Optical-resolution Photoacoustic Microscopy
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Cited by 27 •
2011
Optical-resolution photoacoustic microscopy (OR-PAM) is an emerging technology capable of imaging optical absorption contrasts in vivo with cellular resolution and sensitivity. Here, we provide a visualized instruction on the experimental protocols of OR-PAM, including system configuration, system alignment, typical in vivo experimental procedures, and functional imaging schemes.
FAQs
Changes in blood oxygenation, blood volume, and light scattering alter the intensity of reflected illumination. Optical reflectance imaging captures these variations across many positions and times, so the resulting signal links optical contrast to activity-related tissue changes rather than directly recording electrical impulses. This makes the method useful for examining hemodynamic components of brain function.
Because it records reflected light over a field, the method can provide wide-area spatial measurements. Electrophysiological and microscopic approaches offer complementary perspectives, so researchers can compare optical patterns with other views of neural tissue and activity. This combined context helps relate cortical organization to activity-related vascular changes without treating one measurement as complete.
Recorded intensity does not represent a single biological variable. Blood oxygenation, blood volume, and light scattering can each contribute to the observed change, producing optical patterns that carry both spatial and temporal information. Treating the signal as a composite response helps researchers interpret maps cautiously when studying brain physiology and neurovascular coupling.
A basic acquisition follows reflected light from tissue to create a map. Illumination interacts with the tissue surface, a camera records the returning intensity across the field, and the measurements are examined over time. Researchers can then identify changes in location and timing that correspond to cortical organization or sensory-evoked activity.
Researchers apply Optical Reflectance Imaging to examine cortical organization and sensory-evoked responses across a broad area. Spatial patterns show where optical changes occur, while temporal information shows when those changes develop. Together, these dimensions help characterize activity-related hemodynamic patterns and provide a tissue-level view of brain physiology.
In neuroscience, the technique is particularly valuable for studying neurovascular coupling, the relationship between neural activity and associated vascular changes. Reflectance variations linked to oxygenation and blood volume can be compared with the location and timing of observed responses. This supports investigations of how brain activity is expressed through hemodynamic changes while complementing electrophysiological and microscopic methods.