Polianhidrit IL-1α Nanopartiküllerinin In vivo Antitümör Aktivitesinin Değerlendirilmesi
1.8K görüntülenme
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1 kez atıf aldı
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09:57 dk.
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28 Haziran 2021
DOI :10.3791/62683-v
28 Haziran 2021
•1.8K görüntülenme
M. M. Hasibuzzaman1,2 , Kathleen A. Ross3,4 , Aliasger K. Salem1,4,5,6 , Balaji Narasimhan3,4 , Andrean L. Simons1,2,3,5,6,7
1Interdisciplinary Graduate Program in Human Toxicology, University of Iowa, 2Department of Pathology, University of Iowa, 3Department of Chemical and Biological Engineering, College of Engineering, Iowa State University, 4Nanovaccine Institute, Iowa State University, 5Division of Pharmaceutics and Translational Therapeutics, College of Pharmacy, University of Iowa, 6Holden Comprehensive Cancer Center, University of Iowa, 7Department of Oral Pathology, Radiology and Medicine, College of Dentistry, University of Iowa
HNSCC'nin sinjenik fare modelinde IL-1α'nın antitümör aktivitesini ve ilişkili toksisitesini incelemek için standart bir protokol tanımlanmıştır.
Chapters in this video
0:04
Introduction
0:55
Preparation and Maintenance of HNSCC Cell Line
2:38
Tumor Implantation
3:14
Blood Collection and Serum Separation
4:20
Multiplexing of Collected Serum
4:51
Collection of Tumor and Inguinal Lymph Node and Preparation of Single-Cell Suspension
5:58
FACS Staining of Single-Cell Suspension
7:42
Results: IL-1α Antitumor Activity in HNSCC Mouse Model
9:03
Conclusion
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