Name: Canlı Dokuda Filizlenen Anjiyogenezin Ex Vivo Mekanik Karakterizasyonu için 2.5D Model (Video) | JoVE Journal
Uploaded: 2025-02-28T13:10:00.000+00:00
Duration: 10 min
Description: Keşfedin, domuz karotis arterinden elde edilen 2.5D ekz vivo modeliyle filizlenen anjiyogenezin mekanik karakterizasyonunu. Traction force mikroskobu kullanılarak hücre mekaniği ve lider-takipçi hücre etkileşimleri incelenmektedir.

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<meta charset="utf8"></head><body>Domuz Arterlerinde Hücresel Filizlenmeyi Analiz Etmek için Çok Yönlü 2.5D Model

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</ol>

<table><tbody><tr><td>% 2 bis-akrilamid</td><td></td><td>Bio-Rad</td><td>1610143</td></tr><tr><td>2-merkaptoetanol</td><td></td><td>Merck Yaşam Bilimleri</td><td>60-24-2</td></tr><tr><td>3- (Trimetoksisilil) propil metakrilat</td><td>Bind-Silan</td><td>Sigma-Aldrich</td><td>440159-100ML</td></tr><tr><td>%40 akrilamid</td><td></td><td>Bio-Rad</td><td>1610140</td></tr><tr><td>Aboslute etanol (analiz için)</td><td></td><td>VWR International</td><td>1.00983.1000</td></tr><tr><td>Mutlak etanol (endüstriyel)</td><td></td><td>VWR International</td><td>83813.41</td></tr><tr><td>Asetik asit, buzul% 100</td><td></td> <td>Merck</td><td>1000562500</td></tr><tr><td>Amonyum persülfat</td><td>APS</td><td>Bio-Rad</td><td>7727-54-0</td></tr><tr><td>antikoru (birincil) - calponin</td><td></td><td>abcam</td><td>ab46794</td></tr><tr><td>antikoru (birincil) - CD31</td><td></td><td>Serotec</td><td>MCA1746</td></tr><tr><td>antikor (birincil) - &alfa;-düz kas aktin</td><td>&alfa;SMA</td><td>Dako</td><td>M0851</td></tr><tr><td>antikoru (ikincil) - keçi-anti-fare-IgG1 Alexa 488</td><td></td><td>Moleküler Problar</td><td>A21121</td></tr><tr><td>antikor (ikincil) - keçi-anti-fare-IgG2a Alexa 555</td><td></td><td>Moleküler Problar</td><td>A21137</td></tr><tr><td>antikor (ikincil) - keçi-anti-tavşan-IgG Alexa 555</td><td></td><td>Moleküler Problar</td><td>A21428</td></tr><tr><td>Otoklav</td><td></td><td>Astell</td><td></td></tr><tr><td>Kalsiyum klorür dihidrat</td><td>CaCl2</td><td>Calbiochem</td><td>208291-250GM</td></tr><tr><td>Kollajen tip I, sıçan kuyruğu</td><td></td><td>Corning</td><td>354236</td></tr><tr><td>Kollajen tip IV, insan plasentası</td><td></td><td>Merck Yaşam Bilimleri</td><td>C5533-5MG</td></tr><tr><td>Endotel Hücre Büyüme Ortamı</td><td>EKG ortamı</td><td>Promocell</td><td>C-22111</td></tr><tr><td>Expoxy kaplı yuvarlak uçlu cımbız</td><td>ince cımbız</td><td>Rubis Pinzette</td><td>E78144-2A</td></tr><tr><td>Floresan kalem, koyu kırmızı</td><td></td><td>Invitrogen</td><td>F8807</td></tr><tr><td>Cam lameller, & Oslash; 13 mm, #1</td><td></td><td>Epredia</td><td>CB00130RA120MNZ0</td></tr><tr><td>Cam lameller, & Oslash; 13 mm, #1.5</td><td></td><td>Epredia</td><td>CB00120RAC20MNZ0</td></tr><tr><td>Hidroklorür asit, %25</td> <td>HCl</td><td>Merck</td><td>1.100316.1000</td></tr><tr><td>Krebs-Henseleit tamponu</td><td></td><td>Sigma-Aldrich</td><td>K3753</td></tr><tr><td>Mikroskop, Leica Application Suite X yazılımı, sürüm 3.5.7.23225</td><td>Leica Microsystems</td><td></td><td></td></tr><tr><td>Mikroskobu, Leica DMi8 epifloresan mikroskop</td><td></td><td>Leica Microsystems</td><td></td></tr><tr><td>Mikroskop, Nikon Ti2 Eclipse</td><td></td><td>Nikon</td><td></td></tr><tr><td>Mikroskop, NIS-Elements AR yazılımı</td><td></td><td>Nikon</td><td></td></tr><tr><td>N,N,N',N'-tetrametiletan-1,2-diamin</td><td>TEMED</td><td>Merck Life Science</td><td>110-18-9</td></tr><tr><td>Nalgene şişesi</td><td></td><td>Thermo Scientific</td><td>2187-0016</td></tr><tr><td>İğne, 21Gx1"</td><td></td><td>Henke Sass Wolf</td><td>HK4710008025</td></tr><tr><td>Normal serum, keçi</td><td></td><td>Gibco</td><td>10098792</td></tr><tr><td>Papaverin hidroklorür</td><td></td><td>Sigma</td><td>61-25-6</td></tr><tr><td>Penisilin / Streptomyosin (10 000 U / mL)</td> <td>P / S</td><td>Gibco</td><td>15140163</td></tr><tr><td>Petri kabı, büyük (145x20mm)</td><td></td> <td>Greiner Bio-one</td><td>639160</td></tr><tr><td>Petri kabı, küçük (60x15mm)</td><td></td><td>Greiner Bio-one</td><td>628160</td></tr><tr><td>Fosfat Tamponlu Tuzlu Tuzlu</td><td>PBS</td><td>Sigma</td><td>P4417</td></tr><tr><td>Pluronic F-127</td><td></td><td>Merck Yaşam Bilimleri</td><td>P2443-250G</td></tr><tr><td>Delinme iğnesi, keskin kapalı uç</td><td></td><td>bilinmiyor</td><td></td></tr><tr><td>Neşter, no. 4</td><td></td><td>Swann-Morton</td><td></td></tr><tr><td>Sodyum hidrojen karbonat</td><td>NaHCO3</td><td>VWR Uluslararası</td><td>144-55-8</td></tr><tr><td>sülfosüksinimidil 6- (4'-azido-2'-nitrofenilamino) heksanoat</td><td>Sulfo-SANPAH</td><td>Thermo Scientific</td><td>22589</td></tr><tr><td>Cerrahi bıçak, no. 20</td><td></td><td>Swann-Morton</td><td></td></tr><tr><td>Cerrahi örtü örtüsü</td><td></td><td>Foliodrape</td><td>2775001</td></tr><tr><td>Cerrahi cımbız</td><td></td><td>Lettix</td><td>400024</td></tr><tr><td>Triton X-100</td><td></td><td>Merck</td><td>9036-19-5</td></tr><tr><td>UV lambası</td><td></td><td>Analytik Jena</td><td>95-0042-13&nbsp;</td></tr><tr><td>kuyulu plaka, 96 oyuklu, F-tabanlı</td><td></td><td>Greiner Bio-one</td><td>655180</td></tr><tr><td>kuyulu plaka, cam tabanlı 12 kuyulu</td><td></td><td>MatTek</td><td>P12G-0-14-F</td></tr></tbody></table>

canlı dokuda filizlenen anjiyogenezin ex vivo mekanik karakterizasyonu için 2.5d model

2.5D Model for Ex Vivo Mechanical Characterization of Sprouting Angiogenesis in Living Tissue

Canlı Dokuda Filizlenen Anjiyogenezin Ex Vivo Mekanik Karakterizasyonu için 2.5D Model

Sprouting angiogenesis is the formation of new blood vessels from pre-existing vasculature and is of great importance for physiological such as tissue ...

25d-model-for-ex-vivo-mechanical-characterization-sprouting

Research

JoVE Journal

Biology

Biyoloji

İndüklenen pluripotent kök hücrelerinden (IPSC-EPs) elde edilen endotel progenatörler, kardiyovasküler hastalık tedavilerinin devrimleştirilmesi ve daha sadık kardiyovasküler hastalık modellerinin oluşturulmasını sağlamak için potansiyele sahiptir. Burada, üç boyutlu (3D) kollajen mikroortamlarda IPSC-EPs kapsülleme ve bu hücrelerin vasculogenik potansiyelinin nicel Analizi açıklanmıştır.

Bir In vitro 3D model ve hesaplamalı boru hattı PSC-türetilen endotel progenitors Vazculogenik potansiyelini ölçmek için

Bu yöntem, standart mikroakışkan bir platformda 40 perfüze 3D mikrokap olarak iPSC kaynaklı endotel hücrelerinin kültürünü açıklar. Bu platform, anastomoz ve anjiyojenik filizlerin ölçeklenebilir ve yüksek iş lenme tarzında stabilizasyonu da dahil olmak üzere, degrade odaklı anjiyojenik filizlenmenin 3Boyutlu olarak incelenmesini sağlar.

IPSC türetilmiş Endotel Hücrelerinin Perfüzyonlu 3D Anjiyojenik Filizlenmesini İn Vitro Olarak İncelemek Için Standart ve Ölçeklenebilir Tsay

Burada, bir mikroakışkan çip ve neovaskülarizasyonun ilk mikroçevresini yeniden sağlayan, endotel hücrelerinin (VC' ler) yüksek luminal kesme stresi, fizyolojik transendotelyal akış seviyesi ve çeşitli vasküler endotelyal büyüme faktörü (VEGF) dağılımı ile aynı anda uyarılmasını sağlayan otomatik olarak kontrol edilen, yüksek verimli bir sirkülasyon mikroakışkan sistemi sağlıyoruz.

Neovaskülarizasyonun İlk Olayını Taklit Etmek için Mikroakışkan Model

Bu tahlil, filizlenen anjiyogenezi in vitro olarak kontrol eden biyolojik süreçleri analiz etmek için 3D-kollajen jel içinde kültürlenmiş embriyoid cisimlere farklılaştırılmış fare embriyonik kök hücrelerini kullanır. Bu teknik, ilaçları test etmek, hastalıkları modellemek ve embriyonik olarak ölümcül olan delesyonlar bağlamında spesifik genleri incelemek için uygulanabilir.

In Vitro Vasküler Hastalık Modellemesi ve İlaç Testi için Fare Embriyonik Kök Hücreleri Kullanılarak Anjiyogenezin Üç Boyutlu Filizlenme Testi

Bu çalışma, iki boyutlu (2D) çizik yara migrasyon testini ve üç boyutlu (3D) küresel filizlenme testini, RNA ekstraksiyonu ve immünositokimya dahil olmak üzere ilgili aşağı akış analiz yöntemleriyle birlikte, in vitro anjiyogenezi incelemek için uygun testler olarak sunmaktadır.

Çizik Yara Migrasyon Testi ve Sferoid Filizlenme Testinden Yararlanarak Anjiyogenezin Fonksiyonel ve Moleküler Düzeyde İncelenmesi

Christopher CW Hughes in vitro anjiyogenez çalışmak için onun kültür sistemi yardımcı programı açıklanır. O dalı, uygun lümen formu ve anastomozlarında tabi kültür damarların endotel hücreleri oluşturmak için kritik bir, henüz tanımlanmamış, çözünür faktörü salgılayan fibroblastlar önemini açıklar.

Christopher Hughes: Bir Anjiyogenez Çalışma in vitro model (Röportaj)

In vitro sistemde aynı anda dört tendon kültür yüklemek için yeni bir tarif edilir.

Tendon ex vivo Mekanik Yükleniyor

Teşhis Araçları Geliştirmek İçin Mikro-Elektro-Mekanik Sistemler (MEMS)

Ekstraselüler matriksin sertliği kuvvetle yapışık hücrelerinin birden fazla davranışları etkiler. Matrix sertliği bir doku boyunca mekansal değişir ve çeşitli hastalık koşullarında değişiklik uğrar. Burada atomik kuvvet mikroskobu microindentation kullanarak fare akciğer dokusunun normal ve fibrotik sertliği mekansal farklılıklar karakterize etmek için yöntemler geliştirmek.

Atomik Kuvvet Mikroskobu kullanarak Akciğer Doku Mikro-Mekanik Karakterizasyonu

Burada, in vivo ve ex vivo spesifik fizyolojik yanıtlarda yer alan nöronal popülasyonların tanımlanması için kullanılan klasik bir teknik olan c-FOS protein immünohistolojik tespitine dayalı bir protokol sunuyoruz.

c-FOS Protein Immünohistolojik Algılama: Belirli Fizyolojik Yanıtları ilgilendim Merkez Pathways bir Marker Olarak Faydalı Aracı<em&gt; İn Vivo</em&gt; ve<em&gt; Ex Vivo</em

Keşfedin, domuz karotis arterinden elde edilen 2.5D ekz vivo modeliyle filizlenen anjiyogenezin mekanik karakterizasyonunu. Traction force mikroskobu kullanılarak hücre mekaniği ve lider-takipçi hücre etkileşimleri incelenmektedir.

Canlı Dokuda Filizlenen Anjiyogenezin Ex Vivo Mekanik Karakterizasyonu için 2.5D Model

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