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荧光标记的果蝇心结构
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JoVE Journal Biology
Fluorescent Labeling of Drosophila Heart Structures
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荧光标记的果蝇心结构

DOI:
10.3791/1423-v

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11:12 min

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October 13, 2009

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, , , , ,
1Biology Department,San Diego State University, 2Development and Aging Program, NASCR Center,The Sanford Burnham Institute for Medical Research

Chapters

  • 00:00Title
  • 00:10Introduction
  • 01:50Preparation and Dissection
  • 06:33Mounting Hearts from Adult Flies
  • 08:15Mounting Larval Hearts
  • 09:35Fluorescent Labeling of Drosophila Heart Structures: Results
  • 10:33Conclusion

Summary

Automatic Translation

Automatic Translation

在这里，我们描述了从幼虫和成虫心管的不同元素的荧光标记的一个基本协议<em>果蝇</em>。这些标本非常适合通过荧光或共聚焦显微镜成像。这种技术允许一个强大的模式生物的心功能的详细的结构分析。

Tags

Fluorescent LabelingDrosophila Heart StructuresDrosophila MelanogasterDorsal VesselCardiomyocytesPericardial CellsAlary MusclesVentral Longitudinal Muscle CellsMyofibrilsCytoskeletal/submembranous Protein ComplexesExtracellular MatrixFixationFluorescent Labeling ProtocolSarcomeric F-actinα-actininMyosin-GFP Expressing Adult FliesPericardinType IV Extracellular Matrix CollagenMounting StrategySemi-intact Adult HeartsImagingConfocal Microscopy

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