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SDS-PAGE/Immunoblot检测人脑皮层组织中的Aβ多聚匀浆使用抗原表位检索
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JoVE Journal Neuroscience
SDS-PAGE/Immunoblot Detection of Aβ Multimers in Human Cortical Tissue Homogenates using Antigen-Epitope Retrieval
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SDS-PAGE/Immunoblot检测人脑皮层组织中的Aβ多聚匀浆使用抗原表位检索

DOI:
10.3791/1916-v

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10:48 min

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April 23, 2010

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, , ,
1Yerkes National Primate Research Center,Emory University, 2Department of Neurology, Institute of Clinical Medicine,Tsukuba University, 3Department of Pathology,New York University School of Medicine, 4Department of Neurology,Emory University

Chapters

  • 00:00Title
  • 00:47Introduction
  • 01:20Preparation of Clarified Tissue Homogenates
  • 02:43SDS-PAGE Sample Preparation and Electrophoresis
  • 04:12Transferring Proteins from Gels to Membranes
  • 05:35Antigen-Epitope Retrieval and Immunoblotting
  • 08:13Aβ Multimers by SDS-Page Analysis Using the 6E10 Antibody
  • 09:53Conclusion

Summary

Automatic Translation

Automatic Translation

我们描述了一个准备的人脑皮层匀浆澄清，经SDS - PAGE分离蛋白，抗原修复，并与Aβ肽抗体免疫印迹技术。使用这个协议，我们一贯检测皮层组织与阿尔茨海默氏症的病理的人类单体和多聚体的Aβ。

Tags

SDS-PAGE/ImmunoblotAβ MultimersAntigen-epitope RetrievalAnomalous FoldingPolymerizationβ-amyloid PeptideNeurodegenerative CascadeAlzheimer’s Disease PathogenesisSelf-aggregationAmyloid FibrilsCerebral Senile PlaquesLow Molecular WeightSoluble Aβ MultimersToxic MultimersProteopathiesSDS-polyacrylamide Gel ElectrophoresisImmunoblottingAβ-specific AntibodiesHighly Concentrated Cortical HomogenatesSmall Pore-size Nitrocellulose MembranesClarified Human Cortical Homogenates

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