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JoVE Journal Neuroscience
İnsan Kortikal Doku Aβ multimerlerin SDS-PAGE/Immunoblot Algılama Antijen-epitop Alma kullanarak homojenatlarında
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İnsan Kortikal Doku Aβ multimerlerin SDS-PAGE/Immunoblot Algılama Antijen-epitop Alma kullanarak homojenatlarında

Article DOI: 10.3791/1916-v • 10:49 min • April 23rd, 2010
April 23rd, 2010 •
1, 2, 3, 1,4
1Yerkes National Primate Research Center, Emory University, 2Department of Neurology, Institute of Clinical Medicine, Tsukuba University, 3Department of Pathology, New York University School of Medicine, 4Department of Neurology, Emory University

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Biz insan kortikal homojenatlarında açıklık, SDS-PAGE ile protein ayırma, antijen alımı ve Aβ peptid antikor immunoblotting hazırlanması için kullanılan bir tekniktir açıklar. Bu protokolü kullanarak, biz sürekli olarak insanlarda Alzheimer patolojisi olan kortikal doku monomerik ve multimer Aβ algılar.

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