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Microinjection of A. aegypti Embryos to Obtain Transgenic Mosquitoes
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Microinjection of A. aegypti Embryos to Obtain Transgenic Mosquitoes

Microinjection of A. aegypti Embryos to Obtain Transgenic Mosquitoes

DOI:
10.3791/219-v

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17:39 min

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July 04, 2007

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, ,
1Department of Molecular Biology and Biochemistry,University of California, Irvine (UCI), 2Department of Molecular Biology and Biochemistry, Department of Microbiology and Molecular Genetics,University of California, Irvine (UCI)

Chapters

  • 00:00Introduction
  • 00:29Embryo Collection
  • 02:26Preparing Microinjection Needles
  • 05:30Alignment and Manipulation of Embryos
  • 08:22Dessication of Embryos
  • 10:18Micromanipulation and Microinjection
  • 13:59Post Injection Treatment of Embryos
  • 15:25Hatching of Larvae
  • 16:50Conclusion

Summary

Automatic Translation

Automatic Translation

In this video, Nijole Jasinskiene demonstrates the methodology employed to generate transgenic Aedes aegypti mosquitoes, which are vectors for dengue fever. The techniques for correctly preparing microinjection needles, dessicating embryos, and performing microinjection are demonstrated.

Tags

MicroinjectionA. Aegypti EmbryosTransgenic MosquitoesDengue FeverNijole JasinskieneMethodologyVectorsTechniquesMicroinjection NeedlesDessicating Embryos

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