A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Introduction
- 00:49Prepare Palmitic Acid Working Solution
- 01:27Applying Palmitic Acid Coating to Microtray
- 02:53Loading the Microtray with Low‐Melt Nematode Growth Media (lmNGM) and Seeding the Microtray Wells with Bacterial Food
- 03:47Enclosing Each Microtray Inside a Single‐Well Plate
- 04:25Adding Worms to the Microtray and Preparing the Culture Environment for Long‐Term Use
- 05:01Imaging Individual Worms in Microtray Wells
- 05:27Results: Correlation of Phenotypes in Individual Animals Across Populations
- 06:53Conclusion
Here, we present a protocol to culture isolated individual nematodes on solid media for lifelong physiological parameter tracking and fluorescence quantification. This culture system includes a palmitic acid barrier around single-worm wells to prevent animals from fleeing, allowing the use of aversive interventions, including pathogenic bacteria and chemical stressors.
