Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Neuroscience
Author Produced

A subscription to JoVE is required to view this content.
You will only be able to see the first 2 minutes.

Production of Chick Embryo Extract for the Cultivation of Murine Neural Crest Stem Cells
 

Production of Chick Embryo Extract for the Cultivation of Murine Neural Crest Stem Cells

Article doi: 10.3791/2380
November 27th, 2010

Summary November 27th, 2010

To cultivate neural crest stem cells (NCSC) in vitro, a special medium (NCSCM) is required. Essential part of NCSCM is chick embryo extract (CEE). We here describe accurate techniques to produce a maximized amount of pure and high quality CEE, including details as the isolation, maceration, centrifugation, and filtration processes.

Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
simple hit counter