Production of Chick Embryo Extract for the Cultivation of Murine Neural Crest Stem Cells

Hello, my name's Annaa from the laboratory of Professor Ika Egar in the Department of Pediatric Oncology at the University Children's Hospital, Esen, Germany. Today we will show you a procedure for preparing extracts of 11 days old prenatal embryonic chicks. Put DMEM on ice to cool it down to at least four degrees Celsius.

We use DMEM glutamate from GIB co prepared sterile tubes by filling them half. With the MEM, wash the eggs with 70%E ethanol use X that have been incubated for 11 days at 100 degree Fahrenheit in humidified incubator. Be sure to let the ethanol be absorbed for at least 30 seconds.