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小鼠神经嵴干细胞的培养鸡胚提取物的生产
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Neuroscience
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JoVE Journal Neuroscience
Production of Chick Embryo Extract for the Cultivation of Murine Neural Crest Stem Cells
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小鼠神经嵴干细胞的培养鸡胚提取物的生产

DOI:
10.3791/2380-v

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10:12 min

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November 27, 2010

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, , , , , ,
1Department of Pediatric Oncology,University Children’s Hospital Essen, 2Department of Developmental Pathology,Bonn Medical School, Institute of Pathology

Chapters

  • 00:01Title
  • 00:19Introduction
  • 00:41Dissection of Chick Embryos
  • 02:54Maceration of Chick Embryos
  • 03:59Centrifugation of Chick Embryos
  • 08:30Conclusions

Summary

Automatic Translation

Automatic Translation

要在体外培养的神经嵴干细胞（NCSC），一种特殊的介质（NCSCM）是必需的。 NCSCM的重要组成部分，是鸡胚提取物（CEE）。在这里，我们描述准确的技术，生产的纯正和高品质的中欧和东欧的最大数额，包括隔离，浸渍，离心，过滤过程的详细信息。

Tags

Chick Embryo ExtractMurine Neural Crest Stem CellsNeuro-ectodermPluripotent Progenitor CellsP75SOX10MigrationSmooth Muscle CellsChromaffin CellsNeuronsGlial CellsMelanocytesCartilageBoneNeural Crest Stem Cell Medium (NCSCM)Growth FactorsChick Embryo Extract PreparationIsolationMacerationCentrifugationFiltration Processes

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