03:23Results: Images of the Grid at Square Magnification with Different Tilt Angles and Hole Exposures and 2D Class Averages Collected at Different Tilt Angles
The present protocol describes a generalized and easy-to-implement scheme for tilted single-particle data collection in cryo-EM experiments. Such a procedure is especially useful for obtaining a high-quality EM map for samples suffering from preferential orientation bias due to adherence to the air-water interface.