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Migratory Behavior of Cells Generated in Ganglionic Eminence Cultures
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Migratory Behavior of Cells Generated in Ganglionic Eminence Cultures

Migratory Behavior of Cells Generated in Ganglionic Eminence Cultures

DOI:
10.3791/2583-v

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06:34 min

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April 21, 2011

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, , ,
1Dept. of Anatomy, Physiology and Genetics,Uniformed Services University, 2Neuroscience Program,Uniformed Services University

Chapters

  • 00:05Title
  • 01:12Reagent Preparation for 3D Tissue Culture
  • 02:22Brain Tissue Dissection and Explant Collection
  • 03:223D Tissue Culture Setup
  • 04:36Live Imaging of 3D Tissue Culture
  • 05:183D Culture Cell Migration Dynamics and Image Analysis
  • 05:59Conclusion

Summary

Automatic Translation

Automatic Translation

Time lapse imaging of 3D tissue culture allows studying migratory behavior of individual cells originating from ganglionic eminence in reaction to fractionated protein extract from cerebral cortex.

Tags

Migratory BehaviorCellsGanglionic Eminence CulturesCerebral CortexExcitatory NeuronsInhibitory NeuronsSubcortical SourcesSpatiotemporal ControlCytoarchitectureConnectivityProliferative ZonesNewborn Ferrets3 Dimensional Culture ArrangementBD Matrigel MatrixGE ExplantsTested ProteinsFluorescent Latex Retrobeads IXLive Imaging

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