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Journal / Neuroscience / Neuromodulation and Mitochondrial Transport: Live …
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Neuroscience

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JoVE Journal Neuroscience
Neuromodulation and Mitochondrial Transport
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Neuromodulation and Mitochondrial Transport: Live Imaging in Hippocampal Neurons over Long Durations

Article DOI: 10.3791/2599-v • 04:51 min • June 17th, 2011
June 17th, 2011 •
*1, *1, *1
1Department of Experimental Neurobiology, The Neurosciences Institute
* These authors contributed equally

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Summary
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We describe a protocol that allows imaging of mitochondria in living neurons via fluorescence microscopy over long durations. Imaging over extended periods is accomplished through lentivirus-mediated expression of a mitochondrially targeted fluorescent protein and use of an inexpensive stage-top incubator that was designed and built in our laboratory.

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Neuromodulation Mitochondrial Transport Live Imaging Hippocampal Neurons Extended Durations Vital Dyes Fluorescent Proteins Cytoskeletal Components Organelles Dynamic Fluorescence Microscopy Rhodamine 123 Mitochondrially Targeted EYFP Primary Neurons Culture Conditions Temperature Humidity PH Fluorescent Signal Signal Intensity Image Analysis Exposure Times Photobleaching Phototoxicity
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