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Chapters
- 00:05Title
- 01:00Introduction
- 01:42Preparation of Lipid Vesicles and aPKC Binding Assays
- 04:28In vitro Lipid-protein Polarity Complex
- 06:10LIMACS Purification of PKCζ-EGFP and the Ceramide Binding Domain C20ζ -EGFP
- 06:59Conclusion
To test the interaction of a protein with its target lipid we used MACS and Annexin V-conjugated magnetic beads and lipid vesicles synthesized from the target lipid and Annexin V-binding phosphatidylserine. Proteins bound to the target lipid are co-purified and analyzed after elution from the beads.
