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Evaluation of Protein–Protein Interactions using an On-Membrane Digestion Technique
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Biochemistry
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JoVE Journal Biochemistry
Evaluation of Protein–Protein Interactions using an On-Membrane Digestion Technique

Evaluation of Protein–Protein Interactions using an On-Membrane Digestion Technique

DOI:
10.3791/59733-v

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07:07 min

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July 19, 2019

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, , , ,
1Division of Biological Chemistry, Department of Molecular Biology,Showa University School of Pharmacy, 2Department of Biochemistry,Showa University School of Medicine

Chapters

  • 00:04Title
  • 00:43Immunoprecipitation
  • 02:42On-membrane Digestion of Proteins
  • 05:41Results: GFP and Calpain-6 Associated Proteins
  • 06:18Conclusion

Summary

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Here, we present a protocol for an on-membrane digestion technique for the preparation of samples for mass spectrometry. This technique facilitates the convenient analysis of protein–protein interactions.

Tags

Keywords: Protein-protein InteractionOn-membrane DigestionProteomic AnalysisImmunoprecipitationMagnetic BeadsPVDF MembraneMass Spectrometry

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