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A Cell Free Assay System Estimating the Neutralizing Capacity of GM-CSF Antibody using Recombinant Soluble GM-CSF Receptor
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Cell Free Assay System Estimating the Neutralizing Capacity of GM-CSF Antibody using Recombinant Soluble GM-CSF Receptor

A Cell Free Assay System Estimating the Neutralizing Capacity of GM-CSF Antibody using Recombinant Soluble GM-CSF Receptor

DOI:
10.3791/2742-v

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07:45 min

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June 27, 2011

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, , , , , , ,
1Bioscience Medical Research Center,Niigata University Medical and Dental Hospital, 2First department of Internal Medicine, School of Medicine,Kyorin University, 3Neosilk Laboratory,Immuno Biological Laboratories Co., Ltd.

Chapters

  • 00:05Title
  • 01:49Production and Purification of sGMRalpha
  • 03:23Biotinylation of Recombinant GM-CSF
  • 04:09A Cell-free GMCSF Receptor-ligand-binding Assay
  • 05:22From Bench to Bedside
  • 07:00Conclusion

Summary

Automatic Translation

Automatic Translation

We designed a cell-free receptor binding assay in order to estimate the binding of granulocyte-macrophage colony-stimulating factor (GM-CSF) to the receptors. It enables us to evaluate competitive inhibition of biotinylated GM-CSF binding to soluble GM-CSF receptor alpha by GM-CSF autoantibody with excellent reproducibility.

Tags

Cell-free Assay SystemNeutralizing CapacityGM-CSF AntibodyRecombinant Soluble GM-CSF ReceptorAutoimmune Pulmonary Alveolar Proteinosis (PAP)Disease SeverityGM-CSF BioactivityNeutralizing Capacity AssessmentGrowth InhibitionHuman Bone Marrow CellsTF-1 CellsBioassay SystemCell-free Receptor-binding AssayTransgenic Silkworm TechnologyRecombinant SGMRαSilk ThreadsCocoons

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