Neuroscience
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Screening Assay for Oxidative Stress in a Feline Astrocyte Cell Line, G355-5
Chapters
Summary July 13th, 2011
A screening method to detect oxidative cellular environments is to measure the oxidation of CM-H2DCFDA. Once oxidized within a cell, CM-H2DCFDA changes from non-fluorescent into a fluorescent compound. This change in fluorescence is measured by flow cytometry and indicates the number of cells in an oxidative environment.
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