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Chapters
- 00:05Title
- 01:25Extracting Collagen from Rat Tails
- 02:513-Dimensional Assay Creation
- 05:14Antibody Staining in Preparation for Optical Projection Tomography
- 07:07Optical Projection Tomography, Reconstruction, and 3D Quantification
- 09:27Representative 3D Constructions
- 10:00Conclusion
We have developed a collagen-based in vitro assay which promotes proliferation and invasion from samples of all breast cancer subtypes. Optical Projection Tomography, a three dimensional microscopy technique was utilised to visualise and quantify tumour expansion. This assay may be used to quantify drug response of individual tumour samples.
Tags
Long-term CultureHuman Breast Cancer SpecimensOptical Projection TomographyBreast CancerMortalityWestern WorldSpread Of Breast CancerPatient PrognosisExperimental SystemsCell LinesPrimary TumoursPleural EffusionsSub-types Of Breast CancersPoor Prognosis Luminal B TumoursTumour MicroenvironmentCulture SystemRat Collagen I CushionsHistologyAdipocytesEpithelial CellsFibroblastsMalignant Epithelial CellsSquamoid MorphologyNuclear PleomorphismMitotic FiguresApoptotic BodiesOptical Projection Tomography (OPT)3D Imaging Technology
