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July 29, 2011
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We have developed a collagen-based in vitro assay which promotes proliferation and invasion from samples of all breast cancer subtypes. Optical Projection Tomography, a three dimensional microscopy technique was utilised to visualise and quantify tumour expansion. This assay may be used to quantify drug response of individual tumour samples.
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Cite this Article
Leeper, A. D., Farrell, J., Dixon, J. M., Wedden, S. E., Harrison, D. J., Katz, E. Long-term Culture of Human Breast Cancer Specimens and Their Analysis Using Optical Projection Tomography. J. Vis. Exp. (53), e3085, doi:10.3791/3085 (2011).
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