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JoVE Journal
Biology

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Long-term Culture of Human Breast Cancer Specimens and Their Analysis Using Optical Projection Tomography
 

Long-term Culture of Human Breast Cancer Specimens and Their Analysis Using Optical Projection Tomography

Article DOI: 10.3791/3085
July 29th, 2011

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Summary July 29th, 2011

We have developed a collagen-based in vitro assay which promotes proliferation and invasion from samples of all breast cancer subtypes. Optical Projection Tomography, a three dimensional microscopy technique was utilised to visualise and quantify tumour expansion. This assay may be used to quantify drug response of individual tumour samples.

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