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Journal / Bioengineering / Organotypische Collagen I Assay: A Temperguss…
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Bioengineering

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JoVE Journal Bioengineering
Organotypische Collagen I Assay
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Organotypische Collagen I Assay: A Temperguss Platform to Cell Verhalten in 3-Dimensional Kontext beurteilen

Article DOI: 10.3791/3089-v • 10:46 min • October 13th, 2011
October 13th, 2011 •
1, 1, 1, 1, 2, 2, 1
1The Beatson Institute for Cancer Research, University of Glasgow, 2Section of Dermatology, School of Medicine, University of Glasgow

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Es wird ein Verfahren zur Herstellung einer 3-dimensionalen Matrix, bestehend aus Kollagen Typ I und primären humanen Fibroblasten beschrieben. Diese organotypischen Gel dient als nützliches Substrat invasive Zellmigration beurteilen, da es grundlegende Funktionen von Gewebestroma nachahmt und ist offen für viele Formen der Mikroskopie.

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Bioengineering Organotypische Kultur Zellmigration Invasion 3-dimensionale Matrix Kollagen I Second Harmonic Generation Host-Tumor-Interaktion Mikroumgebung
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