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Subcutaneous Administration of Muscarinic Antagonists and Triple-Immunostaining of the Levator Auris Longus Muscle in Mice
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Neuroscience
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JoVE Journal Neuroscience
Subcutaneous Administration of Muscarinic Antagonists and Triple-Immunostaining of the Levator Auris Longus Muscle in Mice

Subcutaneous Administration of Muscarinic Antagonists and Triple-Immunostaining of the Levator Auris Longus Muscle in Mice

DOI:
10.3791/3124-v

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07:09 min

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September 08, 2011

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, ,
1Biology Department,Arcadia University, 2Shriners Hospitals Pediatric Research Center,Temple University School of Medicine, 3Shriners Hospitals Pediatric Research Center and Department of Anatomy and Cell Biology,Temple University School of Medicine

Chapters

  • 00:05Title
  • 01:28Subcutaneous Administration of mAChR Antagonists
  • 02:51Dissection of LAL Muscles
  • 04:40Triple Immunostaining of LAL NMJs: Day 2
  • 05:33The Effects of a Muscarinic Antagonist on NMJ Structure
  • 06:49Conclusion

Summary

Automatic Translation

Automatic Translation

We describe procedures for repeated administration of inhibitors of muscarinic signaling to the levator auris longus (LAL) muscle of young adult mice and for subsequent immunostaining of its neuromuscular junctions (NMJs) in wholemounts. The LAL muscle has unique advantages for revealing in vivo pharmacological effects on NMJs.

Tags

Subcutaneous AdministrationMuscarinic AntagonistsTriple-immunostainingLevator Auris Longus MuscleMiceHind Limb MusclesIn Vivo Pharmacological StudiesNMJsDrug PenetrationSubcutaneous AdministrationIntramuscular AdministrationSystemic AdministrationMini-pumpsInflammatory ResponsesDirect Muscle DamageHind Limb Muscle AnalysisSerial SectioningExtensive ImmunostainingMouse LAL MusclePinna MovementFacial Nerve BranchStylomastoid Foramen

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