JoVE Encyclopedia of Experiments
Neuroscience
0 views • 3:09 min • July 8th, 2025
Take a mouse brain slice containing the medial thalamus, or MT, projecting to the anterior cingulate cortex, or ACC.
Secure the slice in a multielectrode array chamber perfused with oxygenated aCSF to record electrical signals.
Place positive and negative electrodes and apply direct current stimulation, or DCS, generating an electric field across the slice.
Disable DCS and place an electrode in the MT. Deliver a stimulus to evoke action potentials in the thalamic neurons, triggering neurotransmitter release.
The neurotransmitters trigger ion influx in ACC neurons and generate electrical signals, termed stimulation-evoked synaptic responses.
Reactivate DCS to monitor its effects on ACC synaptic responses.
Next, deactivate DCS and introduce seizure-inducing drugs.
The drugs increase neuronal excitability, causing the thalamic stimulus to produce continuous bursts of signals in the ACC, termed evoked epileptic seizures.
Finally, reactivate DCS to evaluate its effect on seizures by measuring the reduction in seizure amplitude and duration.
In this procedure, ensure the generation of uniform electric fields by passing currents between two parallel silver chloride-coated silver wires tha
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