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JoVE Journal
Biology

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Quantifying Agonist Activity at G Protein-coupled Receptors
 

Quantifying Agonist Activity at G Protein-coupled Receptors

Article DOI: 10.3791/3179
December 26th, 2011

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Summary December 26th, 2011

A method for estimating the affinity constant of an agonist for the active state (Kb) of a G protein-coupled receptor is described. The analysis provides absolute or relative measures of Kb depending on whether constitutive receptor activation is measurable. Our method applies to various responses downstream from receptor activation.

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