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Flash fotolyse af Caged forbindelser i Cilia af olfaktoriske sensoriske neuroner
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Chapters
- 00:05Title
- 00:08Introduction
- 01:35Instrumentation
- 03:37Dissociation of Olfactory Sensory Neurons
- 07:51Recording
- 09:37Representative Results
- 11:09Conclusion
Fotolyse i bur forbindelser tillader produktion af hurtige og lokaliserede stigninger i koncentrationen af forskellige fysiologisk aktive stoffer. Her viser vi hvordan du får patch-clamp optagelser kombineret med fotolyse i bur cAMP eller anbragt i bur Ca for studiet af olfaktoriske transduktion i dissocierede mus lugtesansen sensoriske neuroner.
Tags
Flash PhotolysisCaged CompoundsOlfactory Sensory NeuronsPatch-clamp RecordingsPhotolysis Of Caged CompoundsOlfactory TransductionDissociated Mouse Olfactory Sensory NeuronsCiliaOdorant BindingReceptorsCAMPCyclic Nucleotide-gated (CNG) ChannelsCa EntryCa-activated Cl ChannelsMouse Olfactory EpitheliumFlash LampUltraviolet FlashesWhole-cell Voltage-clamp Configuration
