JoVE Encyclopedia of Experiments
Neuroscience
0 views • 3:18 min • August 7th, 2025
Take an anesthetized mouse that has been fed an inhibitor-containing diet.
In the brain tissue, this inhibitor selectively eliminates microglial cells.
Apply a hyaluronidase solution to both nostrils.
Place the mouse briefly in the supine position to prevent drainage of the solution.
Hyaluronidase breaks down the extracellular matrix of the nasal mucosa, loosening the cells and enhancing tissue penetration.
Administer induced pluripotent stem cell-derived human microglia or iPSMG cell suspension into one nostril.
Place the mouse in the supine position. Then, administer the suspension into the other nostril.
Repeat the administration several times.
The iPSMG cells penetrate the nasal mucosa and follow the olfactory pathway to reach the brain, thereby bypassing the blood-brain barrier.
Allow the mouse to recover.
Anesthetize the mouse and administer transplantation media intranasally at regular intervals.
Growth factors in the media support the growth and survival of transplanted iPSMG cells in the mouse brain.
To prepare mice for transnasal transplantation, feed the male mice with a diet containing PLX for seven days. At the end of the seventh day, seize the PLX diet and feed the mice with a n
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