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Enkelt Les og Sammenkoblede Slutt mRNA-Seq Illumina Bibliotekene fra 10 nanogram Total RNA
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Enkelt Les og Sammenkoblede Slutt mRNA-Seq Illumina Bibliotekene fra 10 nanogram Total RNA

Article DOI: 10.3791/3340-v • 14:50 min • October 27th, 2011
October 27th, 2011 •
1, 1, 1, 1, 1,2,3, 1, 1
1Regenerative Biology, Morgridge Institute for Research, 2Department of Cell & Regenerative Biology, University of Wisconsin, 3Department of Molecular, Cellular, & Regenerative Biology, University of California

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Her beskriver vi en metode for utarbeidelse av både single lese og koblet end Illumina mRNA-Seq sekvensering biblioteker for genekspresjonsanalyse basert på T7 lineære RNA forsterkning. Denne protokollen krever bare 10 nanogram av å starte total RNA og genererer svært konsistente bibliotekene representerer hele transkripsjoner.

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Molecular Biology Genetics mRNA-Seq Illumina-Seq genekspresjon profilering high throughput sekvensering
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