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JoVE Journal
Bioengineering

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A Toolkit to Enable Hydrocarbon Conversion in Aqueous Environments
 

A Toolkit to Enable Hydrocarbon Conversion in Aqueous Environments

Article doi: 10.3791/4182
October 2nd, 2012

Summary October 2nd, 2012

A sustainable auto regulating bacterial system for the remediation of oil pollutions was designed using standard interchangeable DNA parts (BioBricks). An engineered E. coli strain was used to degrade alkanes via β-oxidation in toxic aqueous environments. The respective enzymes from different species showed alkane degradation activity. Additionally, an increased tolerance to n-hexane was achieved by introducing genes from alkane-tolerant bacteria.

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