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JoVE Journal
Bioengineering

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JoVE Journal Bioengineering
A Toolkit to Enable Hydrocarbon Conversion in Aqueous Environments
On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids
Journal (Bioengineering)
On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids
Synthesis of Hydrogels with Antifouling Properties As Membranes for Water Purification
Journal (Bioengineering)
Synthesis of Hydrogels with Antifouling Properties As Membranes for Water Purification
 

A Toolkit to Enable Hydrocarbon Conversion in Aqueous Environments

Article DOI: 10.3791/4182
October 2nd, 2012 •
1, 1, 1, 1, 2, 1
1Department of Biotechnology, Delft University of Technology, 2Delft Center for Systems and Control, Delft University of Technology

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October 2nd, 2012

A sustainable auto regulating bacterial system for the remediation of oil pollutions was designed using standard interchangeable DNA parts (BioBricks). An engineered E. coli strain was used to degrade alkanes via β-oxidation in toxic aqueous environments. The respective enzymes from different species showed alkane degradation activity. Additionally, an increased tolerance to n-hexane was achieved by introducing genes from alkane-tolerant bacteria.

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