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A Toolkit to Enable Hydrocarbon Conversion in Aqueous Environments
A Toolkit to Enable Hydrocarbon Conversion in Aqueous Environments
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
A Toolkit to Enable Hydrocarbon Conversion in Aqueous Environments

A Toolkit to Enable Hydrocarbon Conversion in Aqueous Environments

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20:28 min

October 02, 2012

DOI:

20:28 min
October 02, 2012

14130 Views
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Summary

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A sustainable auto regulating bacterial system for the remediation of oil pollutions was designed using standard interchangeable DNA parts (BioBricks). An engineered E. coli strain was used to degrade alkanes via β-oxidation in toxic aqueous environments. The respective enzymes from different species showed alkane degradation activity. Additionally, an increased tolerance to n-hexane was achieved by introducing genes from alkane-tolerant bacteria.

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