A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 02:33BioBrick Assembly
- 03:37Alkane Converstion Resting Cell Assay, in vivo
- 04:46Alkane Conversion Enzyme Assay, in vitro
- 06:18Ethyl Acetate Hydrocarbon Extraction and Concentration Measurements
- 07:40Alcohol/Aldehyde Dehydrogenase Activity Assay
- 08:49pCaiF Characterization and Tolerance Assay
- 10:26Homolog Interaction Mapping
- 11:11Representative Hydrocarbon Conversion Results
- 19:46Conclusion
A sustainable auto regulating bacterial system for the remediation of oil pollutions was designed using standard interchangeable DNA parts (BioBricks). An engineered E. coli strain was used to degrade alkanes via β-oxidation in toxic aqueous environments. The respective enzymes from different species showed alkane degradation activity. Additionally, an increased tolerance to n-hexane was achieved by introducing genes from alkane-tolerant bacteria.
Tags
Hydrocarbon ConversionAqueous EnvironmentsEscherichia ColiToolkitAlkanesBioBricksGene Expression RegulationSurvival In Toxic EnvironmentsAlkane Degradation PathwayAlkanolsAlkanalsAlkanoic-acidsβ-oxidation PathwayMedium-chain AlkanesCycloalkanesAlkane Hydroxylase SystemGordonia Sp. TF68Long-chain AlkanesGeobacillus ThermodenitrificansADHALDHEnzyme ActivityGlucose ConditionsSubstrate-regulated Promoter
