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Light-driven Enzymatic Decarboxylation

JoVE Journal
Chemistry

This content is Free Access.

JoVE Journal Chemistry

Light-driven Enzymatic Decarboxylation

Light-driven Enzymatic Decarboxylation

DOI:

10.3791/53439-v

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09:58 min

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May 22, 2016

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Katharina Köninger, Marius Grote, Ioannis Zachos, Frank Hollmann, Robert Kourist

¹Faculty of Biology and Biotechnology,Ruhr Universität Bochum, ²Biology and Biotechnology,Delft University of Technology

Chapters

00:05Title
01:00Production of Recombinant Decarboxylase in Escherichia coli
03:42Purification of OleT_JE
05:22Biotransformation of Fatty Acids Using OleT
06:50Analysis of Biotransformation Products
08:26Results: Analysis of the Light-catalyzed Biotransformations by Gas Chromatography
09:19Conclusion

Summary

Automatic Translation

We describe a protocol for the light-catalyzed generation of hydrogen peroxide — a cofactor for oxidative transformations.

Tags

Keywords: Enzymatic Decarboxylation Light-driven Biocatalysis OleT Enzyme Fatty Acid Decarboxylation Terminal Alkyne Synthesis Hydrogen Peroxide Generation E. Coli Expression Protein Purification Cell Lysis Cell-free Extract

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