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Chapters
- 00:05Title
- 02:02Preparation of Instruments, Culture Media and Dishes
- 04:19Selection of Embryos
- 06:36Preparation of Explants
- 10:01Harvesting Explants for Analysis
- 10:39Analysis of Gene and Protein Expression: Representative Results
- 13:03Conclusion
The fate of an individual embryonic cell can be influenced by inherited molecules and/or by signals from neighboring cells. Utilizing fate maps of the cleavage stage Xenopus embryo, single blastomeres can be identified for culture in isolation to assess the contributions of inherited molecules versus cell-cell interactions.
