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Chapters
- 00:05Title
- 01:42Bone Marrow Cell Isolation
- 04:34Competitive Bone Marrow Transplantation, Sample Collection, and Genomic DNA Isolation
- 06:25Standard Curve Preparation and Real-time PCR
- 07:47Representative Donor Cell Engraftment Results
- 09:46Conclusion
Determining donor cell engraftment presents a challenge in mouse bone marrow transplant models that lack well-defined phenotypical markers. We described a methodology to quantify male donor cell engraftment in female transplant recipient mice. This method can be used in all mouse strains for the study of HSC functions.
