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Using Microfluidics Chips for Live Imaging and Study of Injury Responses in Drosophila Larvae
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Using Microfluidics Chips for Live Imaging and Study of Injury Responses in Drosophila Larvae

Using Microfluidics Chips for Live Imaging and Study of Injury Responses in Drosophila Larvae

DOI:
10.3791/50998-v

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11:46 min

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February 07, 2014

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, , , , , , ,
1Department of Molecular, Cellular and Developmental Biology,University of Michigan, 2Department of Biomedical Engineering,University of Michigan, 3Life Sciences Institute,University of Michigan, 4Department of Cell and Developmental Biology,University of Michigan, 5Department of Mechanical Engineering,University of Michigan

Chapters

  • 00:05Title
  • 01:47Preparing the PDMS Chip
  • 03:47Using the Larva Chip for Live Imaging
  • 07:23Inducing a Nerve Crush Injury to Larval Segmental Nerves
  • 08:42Immobilization Enhanced Imaging
  • 10:25Conclusion

Summary

Automatic Translation

Automatic Translation

Drosophila larvae are an attractive model system for live imaging due to their translucent cuticle and powerful genetics. This protocol describes how to utilize a single-layer PDMS device, called the 'larva chip' for live imaging of cellular processes within neurons of 3rd instar Drosophila larvae.

Tags

Keywords: MicrofluidicsDrosophila LarvaeLive ImagingInjury ResponsePDMS MicrochamberImmobilizationNerve CordSegmental NervesBody Wall MusclesHigh-resolution ImagingAnestheticsPhysiological ProcessesLongitudinal StudiesAxon TransportCalcium ResponsesPhoto-convertible ProteinsAxonal InjuryNerve Crush

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