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Drosophila Embryo Preparation and Microinjection for Live Cell Microscopy Performed using an Automated High Content Analyzer
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Drosophila Embryo Preparation and Microinjection for Live Cell Microscopy Performed using an Automated High Content Analyzer

Drosophila Embryo Preparation and Microinjection for Live Cell Microscopy Performed using an Automated High Content Analyzer

DOI:
10.3791/61589-v

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05:52 min

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January 19, 2021

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, ,
1Department of Biology,San Francisco State University, 2Department of Biochemistry & Biophysics,UCSF Mission Bay

Chapters

  • 00:05Introduction
  • 00:26Mounting Embryos on Coverslips
  • 02:06Desiccating and Microinjecting Embryos
  • 04:17Results: Rtnl1-GFP and ReepB-GFP Enrichment at the Spindle Poles During Mitosis
  • 05:03Conclusion

Summary

Automatic Translation

Automatic Translation

Presented here is a protocol to microinject and simultaneously image multiple Drosophila embryos during embryonic development using a plate-based, high content imager.

Tags

Keywords: DrosophilaEmbryoMicroscopyLive CellHigh Content AnalyzerSample PreparationMicroinjectionMulti-point AcquisitionEgg PickerHeptane GlueHalocarbon OilMicroinjection NeedleRazor BladeSpatulaCoverslipFour-slide Plate AdapterMicroinjector

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