Immunohistochemical and Calcium Imaging Methods in Wholemount Rat Retina

Allison Sargoy; Steven Barnes; Nicholas C. Brecha; Luis P&#233;rez De Sevilla M&#252;ller

doi:10.3791/51396

Journal

/

Neuroscience

/

Immunohistochemical and Calcium Imaging Methods in Wholemount Rat Retina

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Immunohistochemical and Calcium Imaging Methods in Wholemount Rat Retina

14,408 Views

•

08:54 min

•

October 13, 2014

DOI:

10.3791/51396-v

DOI:

10.3791/51396-v

•

08:54 min

October 13, 2014

•

4 Views

Allison Sargoy¹, Steven Barnes^1,2,3, Nicholas C. Brecha^1,2,4, Luis Pérez De Sevilla Müller¹

¹Department of Neurobiology,University of California, Los Angeles, ²Veterans Administration Greater Los Angeles Healthcare System, ³Departments of Physiology & Biophysics and Ophthalmology & Visual Sciences,Dalhousie University, ⁴Departments of Neurobiology and Medicine, Jules Stein Eye Institute, CURE-Digestive Diseases Research Center, David Geffen School of Medicine,University of California, Los Angeles

Summary

Immunohistochemistry protocols are used to study the localization of a specific protein in the retina. Calcium imaging techniques are employed to study calcium dynamics in retinal ganglion cells and their axons.

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Immunohistochemical and Calcium Imaging Methods in Wholemount Rat Retina

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