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Immunohistochemical and Calcium Imaging Methods in Wholemount Rat Retina
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Neuroscience
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JoVE Journal Neuroscience
Immunohistochemical and Calcium Imaging Methods in Wholemount Rat Retina

Immunohistochemical and Calcium Imaging Methods in Wholemount Rat Retina

DOI:
10.3791/51396-v

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08:54 min

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October 13, 2014

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, , ,
1Department of Neurobiology,University of California, Los Angeles, 2Veterans Administration Greater Los Angeles Healthcare System, 3Departments of Physiology & Biophysics and Ophthalmology & Visual Sciences,Dalhousie University, 4Departments of Neurobiology and Medicine, Jules Stein Eye Institute, CURE-Digestive Diseases Research Center, David Geffen School of Medicine,University of California, Los Angeles

Chapters

  • 00:05Title
  • 01:25Eyecup Preparation
  • 02:58Wholemount Retina Preparation and Processing
  • 05:06Calcium Imaging in Retinal Ganglion Cells
  • 07:19Results: RGC Immunostaining and Electrophysiology
  • 08:29Conclusion

Summary

Automatic Translation

Automatic Translation

Immunohistochemistry protocols are used to study the localization of a specific protein in the retina. Calcium imaging techniques are employed to study calcium dynamics in retinal ganglion cells and their axons.

Tags

Keywords: ImmunohistochemistryCalcium ImagingWholemount RetinaRetinal Ganglion CellsVoltage-gated Calcium ChannelsDisplaced Amacrine Cells

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