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Method for Obtaining Primary Ovarian Cancer Cells From Solid Specimens

Article DOI: 10.3791/51581-v • 05:58 min • February 4th, 2014
February 4th, 2014 •
1, 1, 1, 2, 1,3, 1,3, 1,3, 1,3
1Department of Obstetrics, Gynecology, and Women's Heath, University of Minnesota, 2Department of Obstetrics and Gynecology, Maricopa Medical Center and St Josephs Hospital and Medical Center, 3Masonic Cancer Center, University of Minnesota

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Summary
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This study describes a detailed method for isolation and characterization of primary ovarian cancer cells from solid clinical specimens. Ovarian cancer clinical specimens are subjected to enzymatic digestion to obtain viable, fibroblast-free epithelial ovarian cancer (EOC) cells highly suitable for downstream applications.

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Primary Ovarian Cancer Cells Solid Specimens Ovarian Cancer Initiation Ovarian Cancer Progression Ovarian Cancer Cell Lines Heterogeneity Genetic Alterations In Vitro Passaging Clinical Specimens Enzymatic Digestion Cell Suspension Downstream Application Drug Screening Representative Specific Clinical Specimens
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